(S)-1-[2-(4-(2-Hydroxy-s-(1-alkylaminopropoxy)phenylalkyl]-4-phenylpiperazines

ABSTRACT

There are disclosed compounds of the formula ##STR1## wherein R 1  is selected from the group consisting of lower alkyl; R 8  is selected from the group consisting of --O--(CH 2 ) n  --wherein n is 2 to 20, ##STR2## and R 6  is selected from the group consisting of hydrogen or lower alkoxy, and ##STR3## wherein R 1  is selected from the group consisting of lower alkyl; R 8  is selected from the group consisting of --O--(CH 2 ) n  --wherein n is 2 to 20, ##STR4## and R 6  is selected from the group consisting of hydrogen or lower alkoxy and racemates thereof. 
     There are also disclosed processes and intermediates utilized to produce the end products. 
     The end products have utility as agents exhibiting both α and selective β adrenergic blocking action.

DESCRIPTION OF THE INVENTION

The present invention relates to binary α,β-Adrenergic Blocking Agentsof the formula ##STR5## wherein R₁ is selected from the group consistingof lower alkyl; R₈ is selected from the group consisting of--O--(CH₂)_(n) -wherein n is 2 to 20, ##STR6## and R₆ is selected fromthe group consisting of hydrogen or lower alkoxy, and ##STR7## whereinR₁ is selected from the group consisting of lower alkyl; R₈ is selectedfrom the group consisting of --O--(CH₂)_(n) -wherein n is 2 to 20,##STR8## and R₆ is selected from the group consisting of hydrogen orlower alkoxy and the racemates thereof.

The presently disclosed and claimed compounds exhibit both α andβ-adrenergic blocking activities which are essential to their use asantihypertensive agents. They provide competitive and reversibleblockade of both α and β adrenoreceptors and have the unexpectedproperty of being cardioselective, having low activity at one site (β₂)and good activity at the β₁ site. This selectivity has importantconsequences when selecting an antihypertensive agent. Further thecompounds have exhibited anti-secretory, i.e. spasmolytic activity.

By the term "lower alkyl" is meant straight or branched chains of C₁ toC₁₀ length with branched chains of C₃ to C₄ as preferred, e.g.,isopropyl or tertiary butyl.

By the term "lower alkoxy" is meant straight or branched chain saturatedhydrocarbonoxy groups containing from 1 to 7 carbon atoms, preferably 1to 4 carbon atoms such as methoxy, ethoxy, propoxy and the like.

The term "halo" or "halogen" refers to all four forms thereof, i.e.,bromine, chlorine, fluorine or iodine with bromine and chlorine aspreferred.

It should be noted that the racemates of the above compounds are alsonovel and exhibit activities similar to the preferred (S) isomersalthough not as quantitatively active. The racemates may also beresolved into the desired isomers when desired.

The following reaction schemes represent the methods of synthesisavailable to produce the novel end compounds of the present invention:##STR9## wherein R₁ is lower alkyl, R₂ and R₃ are selected from thegroup consisting of hydrogen, mesyl, tosyl, brosyl or benzenesulfonyland R₄ is selected from the group consisting of halo, mesyloxy ortosyloxy ##STR10## wherein R₅ is halo ##STR11## wherein R₁ and R₃ are asabove, R₆ is hydrogen or lower alkoxy, n is 2 to 20 and X is halo##STR12## wherein R₁ and R₆ are as above and n is 2 to 20 ##STR13##wherein R₁ and R₆ are as above and n is 2 to 20 ##STR14## wherein R₁ andn are as above and R₇ is halo ##STR15## wherein R₁, R₆ and n are asabove ##STR16## wherein R₁ and R₆ are as above ##STR17## wherein R₁, R₆and n are as above D-Mannitol→I

The compound of formula I which is a known compound is produced byutilizing an acid catalyzed ketal exchange reaction. The reaction iscarried out utilizing a strong mineral acid, such as, sulfuric acid orp-toluenesulfonic acid or a cation exchange resin. The reaction iscarried out at a temperature range of about 0° C. to 100° C. with roomtemperature as preferred. The time of the reaction to completion willvary from 1 to 16 hours depending on the reaction temperature selected.

I→II→V

The two step reaction to produce the compound of formula V is a knownreaction, see, for example, J. Lecocq and C. E. Ballou, Biochemistry, 3,976, (1964).

V→VI

The compound of formula VI is produced by reaction of the primaryalcohol (V) with a lower alkyl or aryl sulfonyl halide in the presenceof a tertiary amine base. Examples of lower alkyl or aryl sulfonylhalides which may be used are mesyl, tosyl, brosyl or benzylsulfonylchlorides or bromides. Examples of tertiary amine bases include pyridineor trialkylamines, e.g. tri-n-butyl or triethylamines. An inert solventmay be utilized to facilitate the reaction such as methylene chloride ortetrahydrofuran or pyridine. The latter functions as both reactant andsolvent. The reaction temperature may vary from about -25° C. to 15° C.with -10° C. to 15° C. as preferable and 0° C. to 15° C. as optimum. Thereaction time may range from 30 minutes to 1 hour depending on thereaction temperature chosen.

VI→IV

The compound of formula IV is thereafter produced by displacement of theleaving group (the alkyl or arylsulfonyloxy group) using a primary aminesuch as a methyl, ethyl, isopropyl or tertiary butyl amine. The reactioncan be performed with or without an inert solvent (benzene, loweralcohols or ethers may be employed). In the case of lower boilingamines, e.g. isopropylamine, the reaction should be run in a pressurizedvessel. The reaction temperature may vary from room temperature to about150° C. with approximately 100° C. as the preferred temperature.

I→II→III→IV

A multistep sequence may also be utilized to produce a compound offormula IV in which the intermediates II and III are not isolated. Thecompound of formula I is oxidized to II utilizing lead tetraacetate inan inert aromatic hydrocarbon solvent, such as, benzene, toluene orxylene. The reaction temperature should be kept at room temperature orbelow, e.g., 0° C. After one side product, Pb(OAc)₂, has been removed byfiltration, the acetic acid which has been generated in the reaction isneutralized by the addition of an alkali metal (Na,K,etc.) carbonate oroxide, e.g., BaO.

The aldehyde (II) is thereafter reacted with a large excess of a primaryamine, e.g. methyl, ethyl, isopropyl, etc. amine to form the imine(III). The reaction temperature should be at about 25° C. or less and adesiccant, e.g. K₂ CO₃, should be used to remove any water formed anddrive the reaction to completion.

Thereafter the imine (III) is hydrogenated to the secondary amine (IV)by the use of a catalyst such as noble metals (Platinum, Palladium,Ruthenium, etc.) on carbon or Raney Nickel/H₂ under pressure. Thisreaction may be carried out at 20° C.-50° C. with room temperature aspreferred. The reaction may be run at 1 to 10 atmospheres depending onthe catalyst chosen.

IV→VII

The amine (IV) is thereafter reacted with an alkyl or aryl sulfonylhalide such as a mesyl, tosyl, brosyl or benzylsulfonyl chloride orbromide in an inert aprotic solvent such as high boiling ethers, e.g.,dioxane, tetrahydrofuran or methylene chloride in the presence of atertiary amine base, e.g., triethyl or trimethylamine. The reaction iscarried out at a temperature range of about -50° C. to 25° C. with about-10° C. to 5° C. as preferred.

VII→VIII

The compound of formula VII thereafter undergoes an acid catalyzedhydrolysis of the ketal protecting group. To effect this catalysis,strong mineral acids are utilized, e.g., HCl, H₂ SO₄ orp-toluenesulfonic acid or a strongly acidic ion exchange resin (H⁺form). The reaction solvent may be water and a miscible co-solvent, suchas, a lower alcohol (methanol, ethanol, propanol, etc.) and ethers suchas tetrahydrofuran or dioxane. The reaction temperature range is fromabout room temperature to 80° C. with about 60° C. to 80° C. aspreferred. The reaction time may range from 1 hour to 2 days dependingon the temperature selected.

VIII→IX

The diol of formula VIII is thereafter reacted with an alkyl or arylsulfonyl halide (as previously disclosed in step V→VI) in the presenceof a tertiary amine base (also disclosed in V→VI) wherein the primaryhydroxyl group is selectively converted into an alkyl or aryl sulfoxygroup. As disclosed previously in step V→VI pyridine may serve as thebase and solvent or other previously disclosed solvents may be utilized(V→VI). The reaction temperature ranges (depending on what sulfonyloxygroup) is desired may vary from about -45° C. to -50° C. (mesyl) to -5°C. to 5° C. (tosyl). When R₄ of formula IX is to be halo the reactionwill differ from above. The compound of formula VIII undergoes an acidcatalyzed exchange reaction with a trialkylorthoacetate, e.g.,trimethyl, triethyl, etc. to give the cyclic orthoacetate of the formula##STR18## and alkanol. The alkanol is distilled from the reactionmixture as formed to facilitate reaction completion. No solvents arenecessary for the reaction. The reaction temperature ranges from about60° C. to 100° C. with 80° C. as preferred. The time of the reactionvaries from 30 minutes to 1 hour depending on the reaction temperature.The cyclic orthoacetate is thereafter reacted with trimethylhalosilanein an inert, aprotic solvent, such as, methylene chloride, giving riseto the intermediate of the formula ##STR19##. which on attack by thehalide ion gives the haloacetate. As solvents for this step, inertaprotic solvents such as high boiling ethers and halogenatedhydrocarbons, e.g., methylene chloride, are best. The reaction may berun from about room temperature to reflux temperature for about 30minutes. A reaction temperature of 40° is preferred. Thereafter thehaloacetate is reacted in an acid catalyzed hydrolysis. To carry out thereaction a solution of the substrate in a hydrolytic solvent such asalcohols, e.g., methanol, ethanol, propanol etc. or aqueous alcoholmixtures containing a catalytic amount of a mineral acid such as HCl, H₂SO₄ or an acidic ion exchange resin are utilized. The reactiontemperature may be varied from 0° C. to reflux temperature (solventdependent) for 30 minutes to 16 hours. Room temperature is preferred.

I→II→V

This procedure is carried out as disclosed previously by a prior artmethod.

V→XI

The hydroxyl group of the compound of formula V is protected as itsbenzyl ether by producing the compound of the formula XI. The reactionis one of the alcohol (V) with an alkali metal hydride, e.g., Na, Li, K,etc., to form the alkoxide which then is reacted with an alkylatingagent, i.e. a benzyl halide (Cl or Br) to give the benzyl ether (XI).Solvents suitable for such a reaction include anhydrousdimethylformamide, dimethylsulfoxide and high boiling ethers such astetrahydrofuran or dioxane. The reaction temperature may range fromabout room temperature to 100° C. with room temperature as preferred.

XI→XII

The compound of formula XII is thereafter produced by an acid catalyzedhydrolysis of the ketal protecting group. Reagents and reactionparameters are the same as in previously disclosed step VII→VIII.

XII→XIII→XIV

The diol of formula XII is converted into the haloacetate of formula XIVvia the compound of formula XIII and the intermediate of the formula##STR20## by following the steps and utilizing the reactants andreaction parameters disclosed previously in step VIII→IX. This series ofreactions are performed because selective alkyl or aryl sulfonyloxy ofthe primary hydroxyl group in XII is difficult due to the similarreactivity of both of the hydroxyl groups in XII.

XIV→XV

The compound of formula XIV thereafter undergoes a two step reactionwherein the acetate (XIV) is saponified to give the intermediatehalohydrin of the formula ##STR21## which is then converted under basicconditions into the epoxide of formula XV. The reaction is carried oututilizing an alkali metal, e.g., Na or K, hydroxide in a solvent of H₂ Oplus an inert water miscible co-solvent such as a lower alcohol, e.g.,methanol, ethanol, propanol, etc. The reaction temperature may vary fromabout -10° C. to 25° C. with a range of about 0° C. to 10° C. aspreferred. It should be noted that the above reactions preserve thestereochemistry of the asymmetric carbon atom throughout.

XVI→XVII

This reaction is a two step sequence wherein a compound of the formulaIX under basic reaction conditions is converted into the epoxide of theformula ##STR22## wherein R₁ and R₃ are as above. This compound acts asthe alkylating agent in the ensuing reaction with the phenol (XVI) underbasic catalysis conditions to form the ether (XVI). The base utilized inthe reaction is an alkali metal hydroxide, e.g., NaOH or KOH and thereaction temperature ranges from about room temperature to 100° C. Thereaction time may vary from about 2 hours to several days depending onthe reaction temperature chosen. The solvents utilized may bedimethylsulfoxide, tetrahydrofuran and lower alcohol/water mixtures.

HYDROXYPHENOL→XVII

This reaction is similar to XVI→XVII except a large excess ofhydroxyphenol is utilized to minimize any dialkylation which mightoccur. As in XVI→XVII the effective alkylating agent is the epoxide (seeabove). The reagents and reaction parameters are as above (XVI→XVII).

XVII→XVIII

The compound of formula XVII thereafter undergoes hydrogenolysis to acompound of formula XVIII. Catalysts for the reaction may be noblemetals such as Platinum, Palladium, Rhodium or Ruthenium on carbon.Suitable solvents include lower alcohols (methanol, ethanol, etc.),esters (ethyl or butyl acetate) and ethers (dioxane or tetrahydrofuran).The reaction temperature may be varied from about 0° C. to 100° C. withroom temperature preferred.

XVI→XIX

This reaction consists of the O-alkylation of the phenol of formula XVIwith a compound of the formula ##STR23## wherein X is a leaving groupselected from the group consisting of halogen, tosyloxy and mesyloxy, R₆is hydrogen or alkoxy and n is 2 to 20, utilizing an alkali metalhydroxide (NaOH, KOH, etc.) in an inert water miscible solvent, e.g., adimethylsulfoxide/H₂ O mixture. The reaction temperature may vary fromabout room temperature to 60° C. with 60° C. as preferred with areaction time from 1-2 hours to several days depending on the reactiontemperature. Alternate O-alkylation systems which may be utilized inconjunction with the phenylpiperazine include alkali metal alkoxides inlower alcohols e.g., sodium methoxide in methanol or potassium carbonatein acetone.

XVIII→XXIII

The compound of formula XVIII is reacted with a compound of the formula

    Y(CH.sub.2).sub.n X

wherein X and Y are the same or different leaving groups with X as aboveand Y selected from the same leaving groups as X.

The reaction which is an O-alkylation of a phenol (XVIII) with anα,ω-dihaloalkane (for example) is carried out utilizing an alkali metalcarbonate, such as, potassium or sodium carbonate in, as preferred,refluxing acetone. The reaction temperature may vary from roomtemperature to reflux with reflux as preferred.

XVIII→XXIV

This reaction follows the same reaction parameters and utilizes the samereagents as previously disclosed in step XVI→XIX.

XVIII→XX

The compound of formula XVIII is thereafter reacted with a compound ofthe formula ##STR24## wherein X is as above and R is lower alkyl.

This O-alkylation of the phenol (XVIII) with the alkyl ω-haloalkanoate,for example, ethyl-6-bromohexanoate or ethyl bromoacetate, using as abase an alkali metal alkoxide, such as, potassium tert-butoxide,methoxide or ethoxide in a lower alcohol, e.g., methanol, ethanol, etc.,at a temperature range of about 0° C. to 100° C. with 60° C. to 80° C.as preferred. The product is subsequently saponified to give the acid(XX). The saponification is usually carried out at between roomtemperature to 65° C. for a period of 3 to 40 hours.

XVIII→XXII

The compound of the formula XVIII is reacted with an alkylating agent ofthe formula ##STR25## wherein X, n and R₆ are as before, such as,1-(ω-haloalkanoyl)-4-phenylpiperazine, in an alcohol/water mixture ortetrahydrofuran or dimethylsulfoxide/water mixture containing an alkalimetal hydroxide, e.g., NaOH or KOH. The reaction is carried out at fromabout room temperature to 100° C. with 75° C.-80° C. as preferred.

XIX→XXI→XXIV

The compound of formula XIX undergoes hydrogenolysis of the benzyl etherportion by utilizing a noble metal catalyst, e.g., Palladium, Platinum,Rhodium, etc. on carbon. The solvent for such a reaction may be analcohol (methanol, ethanol, etc.) or acetic acid containing a smallamount of mineral acid such as HCl or H₂ SO₄. The reaction temperaturemay vary from about 0° C.-100° C. with room temperature as preferred.The compound of formula XXI thereafter undergoes an O-alkylation usingan alkylation using an alkylating agent (IX) in the presence of analkali metal hydroxide as a base. The reagents and reaction parametersfor this reaction are as previously disclosed for step XVI→XVII.

XX→XXII

The acid of the formula XX is converted to the activated intermediate##STR26## wherein R₁, R₃ and n are as above on treatment with ethylchloroformate under anhydrous conditions in an aprotic solvent, e.g.tetrahydrofuran or dioxane, at a low temperature e.g., about 0° C. to 5°C. in the presence of a tertiary amine base, e.g. trialkylamine. Thismixed anhydride is treated in situ with a phenylpiperazine of thegeneral formula ##STR27## wherein R₆ is as above to give the tertiaryamide. This reaction is carried out at between about 10° C. to 25° C.

XXIV→XXV

The compound of formula XXIV undergoes a reductive cleavage of the -R₃protecting group utilizing as the reducing agent a 60-70% solution ofsodium bis-methoxyethoxy aluminum hydride in an inert aromatichydrocarbon solvent such as benzene or toluene. Inert aprotic solventssuch as tetrahydrofuran or dioxane may also be utilized. The reactionmay be carried out at from about room temperature to 100° C. with arange of about 80° C. to 100° C. as preferred.

XXII→XXV

The compound of formula XXII undergoes a reductive cleavage of the -R₃protecting group with a concommitant reduction of the amide function toan amino group. The reagents and reaction parameters for this reactionare as previously disclosed in step XXIV→XXV with the exception that aproportionately greater amount of the hydride reducing agent isemployed.

XV→XXX

Suitably protected the optically active 2,3-epoxypropanol (XV) isthereafter reacted with a substituted phenol of the formula XVI in anO-alkylation using an alkali metal alkoxide, e.g., sodium or potassiummethoxide or ethoxide, as the base in a lower alcohol solvent (methanol,ethanol, etc.). Also useful as a base in the above reaction would be analkali metal hydroxide, e.g., NaOH or KOH in an aqueous alcohol,tetrahydrofuran, dioxane or dimethylsulfoxide solvent. The reactiontemperature may range from room temperature to reflux for the chosensolvent with 60° C.-80° C. as preferred.

XXX→XXXI

The benzyl ethers of the formula XXX thereafter undergo a hydrogenolysisutilizing a catalyst of a noble metal such as Palladium, Platinum,Ruthenium etc. on carbon. Suitable solvents include alcohols (methanol,ethanol, propanol, etc.) or acetic acid containing a small amount of amineral acid such as H₂ SO₄ or HCl. The reaction temperature may rangefrom about 0° C. to 100° C. with room temperature as preferred. Thehydrogenolysis may also be run under pressure up to 10 atmospheres if acatalyst such as H₂ /Raney Nickel is chosen.

XXXI→XXXII→XXXIII

The reagents and reaction parameters for this step have been previouslydisclosed in step XII→XIII→XIV.

XXXIII→XXXIV

The acetate (XXXIII) thereafter undergoes an acid catalysed hydrolysiswherein the acetate reacts in a hydrolytic solvent, such as, alcohols,e.g., methanol, ethanol, etc. or alcohol/water mixtures which contain acatalytic amount of a mineral acid such as H₂ SO₄ or HCl or an acidicion exchange resin. The reaction may be carried out at from about 0° C.to reflux of the chosen solvent with a range of about room temperatureto 60° C. as preferred.

XXXIV→XXXV

The halohydrin (XXXIV) is therefore reacted with a phenylpiperazine ofthe formula ##STR28## wherein X is halo.

The reaction involves two separate and unrelated baseinducedtransformations, i.e., (1) conversion of the halohydrin into an epoxideand (2) the O-alkylation of the phenol with the selectedphenylpiperazine. The reaction is carried out under basic conditionsutilizing an alkali metal hydroxide, e.g., NaOH or KOH in an aqueousdimethylsulfoxide, tetrahydrofuran or alcohol (methanol, ethanol, etc.)solvent. The reaction is carried out from about 0° C. to 40° C. withhigher temperatures, e.g., 40° C. as preferred.

XXXV→XXV

The epoxide of formula XXXV is reacted with a monoalkylamine such asisopropyl, t-butyl, etc. amine to produce the amino alcohol. Solventsfor such a reaction are alcohols (C₁ -C₄) with methanol preferred orethers, such as, tetrahydrofuran or dioxane. The reaction may be runfrom about 0° C. to 100° C. with about 65° C. as preferred.

XVII→XXXVI

The compound of formula XVII undergoes a reductive cleavage of theN-protected amine to give the secondary amine. Reagents and reactionparameters for this reaction have been previously disclosed for stepXXIV→XXV.

XXXVI→XXXVII

The benzyl ether of formula XXXVI thereafter undergoes hydrogenolysis ina catalyzed reaction. Suitable catalysts include noble metals (aspreviously disclosed) on carbon in a solvent such as lower alcohols (C₁to C₄) e.g., methanol or ethanol at a temperature range of from about 0°C. to 100° C. If H₂ /Raney Nickel is chosen as the catalyst, thereaction should be run under pressure, e.g., up to 10 atmospheres.

XXXVII→XXV

The phenol (XXXVII) undergoes a base induced O-alkylation with aphenylpiperazine of the formula ##STR29## wherein n and R₆ are as above.in an aqueous dimethylsulfoxide, tetrahydrofuran or dioxane solvent.Suitable bases include alkali metal hydroxides, such as, NaOH or KOH.The reaction temperature ranges from about 0° C. to 100° C. with about60° C. as preferred.

XLII→XLIII

The compound of the formula XLII is reacted with a compound of theformula ##STR30## to produce the compound of the formula XLIII. Thereactants and reacted parameters have been previously disclosed in stepXV→XXX.

XLIII→XLIV

The compound of formula XLIII thereafter undergoes a hydrogenolysis aspreviously disclosed and with the same reagents and reaction parametersas step XXX→XXXI.

XLIV→XLV→XLVI

The compound of formula XLIV is converted to a compound of formula XLVIutilizing the same reagents and reaction parameters as previouslydisclosed in step XII→XIII→XIV.

XLVI→XLVII

The compound of formula XLVI undergoes a three step reaction under basicconditions as follows:

(A) saponification of the methyl ester

(B) saponification of the acetate to give the halohydrin

and

(C) conversion of the intermediate halohydrin into the epoxide (XLVII).As the base, an alkali metal hydroxide, e.g., NaOH or KOH, in a solventof water plus an inert water miscible co-solvent such as a lower alcohol(C₁ to C₄) e.g., methanol or tetrahydrofuran or dioxane. The reaction iscarried out from about 0° C. to 30° C. with a preferred temperaturerange of about 10° C. to 25° C.

XLVII→XLVIII

The activated ester of formula XLVIII is produced by treatment of theacid (XLVII) with an excess of a haloacetonitrile (Halo - CH₂ C.tbd.N)in the presence of a tertiary amine base, such as, trialkylamine, e.g.,triethyl-or trimethylamine. The reaction temperature may range fromabout 0° C. to 70° C. with a range of about 25° C.-70° C. as preferred.The activated ester may be utilized to undergo condensation reactions,such as reactions with amines to form amides at a much faster rate thanthe ordinary methyl or ethyl esters.

XLVIII→XLIX

The ester of formula XLVIII is thereafter condensed with a primary amineof the formula ##STR31## to form the amide (XLIX). An inert solvent,such as, tetrahydrofuran or dioxane, may be utilized at a temperaturerange of about 0° C. to 100° C. with room temperature as preferred.

XLIX→L

The epoxide of formula XLIX is thereafter reacted with a primary aminee.g., an amine of the formula

    NH.sub.2 -R.sub.1

wherein R₁ is as above in a solvent, such as, C₁ to C₄ alcohols orethers, e.g., tetrahydrofuran or dioxane, to form the amino alcohol (L).The reaction may be run at from about 0° C. to reflux temperature withroom temperature to 65° C. as preferred.

LI→LII

The alkene portion of the formula LI* compound is reacted with ahypohalous acid (generated in situ from an N-Halosuccinimide in aqueousacetone containing a catalytic amount of HClO₄) to give a mixture ofhalohydrins, i.e., LII and the compound of the formula ##STR32##

LII→LIII

The bromohydrins (LII and LII') are thereafter converted under basicconditions to the epoxide (LIII). As a base, an alkali metal (Na, K,etc.) hydroxide may be utilized. Suitable solvents for the reactioninclude ethers, such as, dioxane or tetrahydrofuran and alcohols (C₁ toC₄) e.g., methanol, ethanol, etc. The reaction can be run at from about0° C. to 40° C. with room temperature as preferred.

LI→LIV

The acid portion of the compound of formula LI is esterified bytreatment of LI with an excess of an alkylating agent, CH₃ -Halo, e.g.,CH₃ I, CH₃ Br, in the presence of an alkali metal (Na, K) carbonate in asolvent, such as, acetone, dimethylformamide, dimethylsulfoxide orhexamethylphosphoramide to give the ester of formula LIV. The reactiontemperature is not critical but about room temperature is preferred forits ease.

LIII→LV

The acid portion of the epoxide (LIII) may also be esterified by usingdiazomethane in the presence of a solvent such as ethers(tetrahydrofuran) or a C₁ to C₄ alcohol. As above, this reaction ispreferably run at room temperature.

LIV→LV

The alkene portion of the compound of formula LIV is thereafter reactedwith an aromatic or aliphatic peracid, such as, m-chloroperbenzoic acid,peracetic acid, performic acid, trifluoroperacetic acid, permaleic acid,perbenzoic acid, monoperphthalic acid, o-sulfoperbenzoic acid orp-nitroperbenzoic acid. The reaction utilizes as a solvent any inerthalogenated aliphatic hydrocarbon, such as, methylene chloride orchloroform. The reaction temperature may range from about 0° C. toreflux with room temperature as preferred.

LV→LVI

The epoxide portion of the compound of formula LV is thereafter reactedwith a primary amine of the formula NH₂ R₁, wherein R₁ is as above, in asuitable solvent, such as, C₁ to C₄ alcohols or ethers, such as, dioxaneor tetrahydrofuran. The reaction temperature may vary from about 0° C.to room temperature with room temperature as preferred.

LVI→LVII

The ester portion of LVI is thereafter condensed with a primary amine ofthe formula ##STR33## wherein n and R₆ are as above, to form the amide.No solvent is necessary for this step. The reaction temperature may befrom about 100° C. to 150° C. with a preferred range of from about 140°C. to 145° C.

XVI→LXII

The phenol portion of XVI undergoes an O-alkylation with anepihalohydrin, e.g., epichlorohydrin, using as a base, an alkali metalhydroxide, e.g. KOH or NaOH in a mixture of H₂ O and dioxane,tetrahydrofuran or dimethylsulfoxide. The reaction may be carried outfrom about 0° C. to 100° C. with about room temperature as preferred.

LXII→LXIII

The epoxide portion of LXII is thereafter reacted with an amine of theformula

    NH.sub.2 R.sub.1

wherein R₁ is as above, in an solvent, such as, a C₁ to C₄ alcohol, oran ether, such as, tetrahydrofuran or dioxane. The reaction temperaturemay range from about 0° C. to reflux with a range of about roomtemperature to 65° C. as preferred.

LXIII→LXIV

The benzyloxy portion of the compound of formula LXIII is converted tothe phenol function by utilizing the reagents and reaction parameterspreviously disclosed for the isomer, see, step XXXVI→XXXVII.

LXIV→XXV'

The phenol portion of LXIV is reacted with a phenylpiperazine aspreviously disclosed in step XXXVI→XXV along with the reagents andreaction parameters.

4-(2-bromoethoxy)phenol*→LVIII

The phenol portion of the bromoethoxy phenol undergoes an O-alkylationwith an alkylating agent, such as, allyl halide, e.g. chloride orbromide, in the presence of an alkali metal (Na,K) carbonate inrefluxing solvent, such as, acetone. The reaction temperature should beabout or at reflux of the solvent.

LVIII→LIX and LIX'

The alkene portion of the compound of formula LVIII is thereafterconverted to the halohydrins of formulas LIX and LIX' by utilizing thereagents and reaction parameters set forth in step LI→LII.

LIX and LIX'→LX

The halohydrins (LIX and LIX') are thereafter converted to the epoxideof formula LX by utilizing the reagents and reaction parameters setforth in step LII→LIII.

LX→LXI

The epoxide (LX) is thereafter reacted with a primary amine of theformula NH₂ -R₁ wherein R₁ is as above, to give the aminoalcohol (LXI).Suitable solvents include C₁ to C₄ alcohols, ethers such astetrahydrofuran or dimethylsulfoxide or dimethylformamide. The reactionmay be carried out from about room temperature to 60° C. with a range ofabout room temperature to 55° C. as preferred.

LXI→XXV'

The halogen portion of LXI is thereafter displaced with a secondaryamine of the formula ##STR34## wherein R₆ is as above to produce acompound of formula XXV'. Solvents suitable for this reaction include C₁to C₄ alcohols and ethers, such as, dioxane and tetrahydrofuran. Thereaction may be carried out at from about 0° C. to 100° C. with 80° C.to 100° C. as preferred.

LXV*→LXVI

A compound of the formula LXV is reacted with an appropriatelysubstituted amine of the formula ##STR35## wherein R₆ is as above in aC₁ to C₄ alcohol. The temperature of the reaction may be varied fromroom temperature to the boiling point of the selected alcohol. Apreferred alcohol is ethanol and the preferred reaction temperature isits boiling point.

LXVI→LXVII

The compound of formula LXVI is thereafter converted to a compound offormula LXVII by treatment with a base such as an alkali or alkalineearth metal hydroxide (NaOH, KOH, Ba(OH₂) in a solvent such as water, C₁to C₄ alcohols or dimethylformamide or alkali metal (K,Na) salts oflower alcohols in solvents such as dimethylformamide or C₁ to C₄alcohols. To this reaction mixture containing the salt of LXVI is addedepihalohydrin to generate LXVII.

LXVII→LXVIII

The compound of formula LXVII is treated with an amine of the formula

    R.sub.1 NH.sub.2

wherein R₁ is as above in a suitable solvent, e.g., C₁ to C₄ alcohols.The reaction may be carried out at from about room temperature to refluxwith about reflux as preferred. A preferred solvent would be methanol.If faster reaction rates are desired, the reaction mixture can be heatedabove its boiling point in a pressurized vessel.

LXIX→LXVIII→LXX

The compound of formula LXVIII can alternatively be prepared from LXIXby treatment with a propenoyl halide in an inert solvent e.g., dioxane,dimethylsulfoxide, etc. at from about 0° C. to 50° C. The intermediateis treated in situ with an appropriately substituted amine of theformula ##STR36## wherein R₆ is as above The reaction conditions are asin step LXV→LXVI above. The compound of formula LXX is generated bymixing maleic acid with LXVIII in an inert solvent.

LXXI→LXXII

The haloalkanoyl halide of formula LXXI is reacted with 4-aminophenol inthe presence of excess 4-aminophenol or an equivalent of a tertiaryamine, such as, pyridine, triethylamine or the like in an inert solvent,such as, an ether, e.g., tetrahydrofuran or dioxane or a polyhalogenatedhydrocarbon, e.g., methylene chloride at a temperature of from about 0°C. to 100° C. A preferred reaction system for this conversion is dioxanein the presence of excess 4-aminophenol at room temperature.

LXXII→LXXIII

The compound of formula LXXII is reacted with an appropriatelysubstituted amine of the formula ##STR37## wherein R₆ is as above in thepresence of a hydrogen halide scavenger, such as excess reagent or aless reactive amine such as triethylamine or pyridine in an inertsolvent e.g., C₁ to C₄ alcohol. The reaction temperature may vary fromabout room temperature to 100° C.

LXXIII→LXXIV→LXXV

Conversion of the compound of formula LXXIII into that of formula LXXVis the same for reagents and reaction parameters as previously disclosedin steps LXVI→LXVII→LXVIII→LXX

The end compounds of the subject invention may be converted to theirpharmaceutically acceptable salts which exhibit comparablepharmacological activity. The end products have three amine functions,but only two of these groups are sufficiently basic to form stablesalts. Accordingly they form diacid salts with various organic andinorganic acids. Some of the useful organic or inorganic acids includemaleic acid, fumaric acid, tartaric acid, citric acid, hydrochloricacid, hydrobromic acid and sulfuric acid. A typical preparation of oneof these salts entails the mixture of a solution of the base endproduct, for example, XXV in a C₁ to C₄ alcohol with a solution of apharmaceutically acceptable acid as outlined above, also in a C₁ to C₄alcohol. The salt thus formed crystallizes spontaneously from solutionor does so on the addition of a suitable co-solvent, for example, ethylacetate, ether, acetone, or halogenated hydrocarbons, such as,chloroform, 1,2-dichloroethane, etc.

An alternative method which may be useful in the case of compounds, suchas the end product L, comprises the treatment of two parts of the basewith an excess of hydrochloric acid in methanol. The solvent is thenremoved in vacuo to drive off the excess acid which gives the unstabletrihydrochloride salt. The salt is then redissolved in methanol and onepart of free base added to the solution. The dihydrochloride salt isthen precipitated from solution on the addition of a co-solvent, such asdisclosed above.

Preferred among the compounds disclosed herein are those of the formula##STR38## wherein R₁ is selected from the group consisting of loweralkyl; R₈ is selected from the group consisting of --O--(CH₂)_(n)-wherein n is 2 to 20, ##STR39## and R₆ is selected from the groupconsisting of hydrogen or lower alkoxy, the racemates thereof andpharmaceutically acceptable salts thereof. Especially preferred arethose compounds wherein R₁ is a branched chain alkyl, such as, isopropylor tertiary butyl, R₈ is the group --O--(CH₂)_(n) -- or ##STR40##wherein n is 2 to 10, most preferably 2, and R₆ is hydrogen.

The compounds of the present invention and their pharmaceuticallyacceptable salts are useful as α and β adrenergic blocking agents whenutilized particularly in oral preparations. As contemplated by thisinvention the novel end products of the present invention and theirpharmaceutically acceptable salts can be embodied in pharmaceuticaldosage formulations containing from about 0.1 to about 50 mgs., mostpreferably 1-50 mg. with dosage adjusted to animal species andindividual requirements. The novel end products and theirpharmaceutically acceptable salts can be administered internally, forexample, parenterally or enterally, in conventional pharmaceuticaldosage forms. For example, they can be incorporated in conventionalpharmaceutical dosage forms. For example, they can be incorporated inconventional liquid or solid vehicles such as water, gelatin, starch,magnesium stearate, talc, vegetable oils and the like to providetablets, elixirs, capsules, solutions, emulsions and the like accordingto acceptable pharmaceutical practices. Although less preferredintravenous and intramuscular delivery systems may be utilized toprovide the above novel compounds.

The disclosed compounds are in the general class of1-aryloxy-3-alkylaminopropan-2-ols, many examples of which have beenshown to possess β-adrenergic blocking activity. Since the class ofcompounds has an asymmetric center there are two enantiomeric forms. Ithas been found that the β-blocking activity of such compounds is to alarge extent found in the isomer having the S-absolute configurationi.e. that isomer that is stereochemically equivalent to the naturallyoccurring β-agonist (R)-(-) epinephrine, whereas the racemic formexhibits approximately half of this activity, see, for example,

(1) R. Howe & B. S. Rao J. Med. Chem. 11, 1118, (1968)

(2) B. Ablad et al Acta. Pharmacol Toxicol. 25, 85 (1967)

(3) L. Almirante & W. Murmann, J. Med. Chem. 9 650 (1966)

(4) M. Dukes & L. H. Smith J. Med. Chem. 14 326 (1971)

(5) J. C. Danilewicz & J. E. G. Kemp 16, J. Med. Chem., 168, (1973)

The desired S-isomer of the disclosed compounds are available in twoways:

(a) by resolution of the racemic material via a fractionalcrystallization of its diastereoisomeric salts formed with an opticallyactive acid, such as tartaric acid.

(b) by asymmetric synthesis using an optically active synthon of theappropriate absolute configuration. Two such synthons, IX and XV, whichare readily available from D-mannitol, a naturally occurring sugar, havebeen used to construct the oxypropanolamine side chain in the disclosedcompounds. Synthon IX is restricted to the syntheses of α, β-blockers,wherein the functionality of the final compound is compatable with theconditions required to remove the amine protecting group, R₃ i.e.reductive cleavage using a mixed metal hydride. When the functionalityof the final compound is not compatable with these conditions, e.g. theamido group in compound L, the synthon XV is used to incorporate theoxypropanolamine side chain.

Applicants, in setting forth the disclosure of the above specificationhave cited the teaching of various articles and U.S. Patents. Suchcitations are meant to incorporate the teachings of these references forcompleteness of disclosure.

The following examples are illustrative but not limitative of thepresent invention. All temperatures are stated in degrees Centigradeunless otherwise indicated.

EXAMPLE 1 (2R,3R,4R,5R)-Mannitol-1,2;5,6-diacetonide.

A mixture of 546 g powdered D-Mannitol (3.0 mol), p-toluenesulfonic acid(3.0 g) and 780 g dimethoxypropane (7.5 mol) in 900 ml dry DMSO wasstirred at ambient temperature under anhydrous conditions. Within 30min-1 hr, all the mannitol had dissolved. After 16 hours the reactionwas poured into 900 ml saturated NaHCO₃ solution and then was dilutedfurther with 2 liters H₂ O. The mixture was extracted with EtOAc (1×4.5l; 3×3 l) and the extracts were washed in turn with H₂ O (3×1.5 l). Thecombined dried (Na₂ CO₃) EtOAc layers were concentrated in vacuo (bathtemp ˜45°) until the residue had solidified. The residue was then heatedto reflux to redissolve the solids and the solution was diluted with ˜8liters of hot hexane. The mixture was allowed to cool slowly overnightand the resulting crystalline material was filtered off and washed withan ether-hexane mixture (1:3; 4×500 ml) to give (2R,3R,4R,5R)-mannitol-1,2;5,6-diacetonide, mp 115°-119°.

The mother liquors were concentrated to dryness. A solution of theresulting residue is ether (300 ml) was diluted with about 1.6 liters ofhexane. This yielded additional diacetonide, mp 119°-120°.

EXAMPLE 2 (2S)-3-Isopropylamino-1,2-propanediol acetonide from(2R,3R,4R,5R)-Mannitol-1,2;5,6-diacetonide via the imine

Pb(OAc)₄ (263 g, 0.59 mol) was dispersed in 1500 ml dry benzene underargon. To the rapidly stirred mixture 140 g of the diacetonide was addedin 5-10 g portions over 15 minutes and then further 1 g portions ofdiacetonide were added until the reaction gave a negative test foroxidant (KI-starch paper). A total of 150 g of acetonide (140 g+10×1 g)was used. The mixture was filtered through Celite and the filter cakewas washed with 2×100 ml portions of dry benzene. The filtrate wasstirred with 300 g anhydrous K₂ CO₃ for 30 minutes to neutralize HOAcwhich was produced in the oxidation. After a second filtration throughCelite, the solution was treated with 450 ml isopropylamine and 300 grof K₂ CO₃. After stirring for 30 min., the mixture was filtered and thefiltrate was hydrogenated over 15 g 10% Pd/C (1 atmos; 23°). Thereaction essentially stopped after the uptake of 26.4 liters of H₂. Thecatalyst was removed by filtration and concentration of the filtratefurnished the amine.

EXAMPLE 3 (2S)-3-N-Mesylisopropylamino-1,2-propanediol acetonide

90 ml (1.2 mol) mesyl chloride was added with stirring to a previouslychilled (-10°) solution of (2S)-3-isopropylamino-1,2-propanediolacetonide (188 g; 1.087 mol) and triethylamine (288 ml; 1.63 mol) in dryTHF at such a rate that the reaction temp did not exceed 5°. Reactionwas then stirred at 10°-15° for 30 min whereupon it was diluted with 1.5l brine. The layers were separated and the aqueous layers were extractedwith ether (3×500 ml). The organic layers were washed in turn with brine(2×500 ml) and then were combined, dried (Na₂ SO₄), and evaporated togive the N-mesylate as an oil.

A small portion was recrystallized (3x) from hexane to give analyticallypure material, mp 33°-34°; [α]_(D) ²⁵ -14.76° (c, 1.0, CHCl₃).

Anal. Calcd. for C₁₀ H₂₁ NO₄ S: C, 47.79; H, 8.42; N, 5.57; S, 12.76.Found: C, 47.87; H, 8.66; N, 5.72; S, 12.89.

EXAMPLE 4 (2S)-3-N-Mesylisopropylamino-1,2-propanediol.

200 ml of prewashed (H₂ O and MeOH) Dowex 50W-8X ion exchange resin (H⁺form) was added to a solution of 264 g crude(2S)-3-N-mesylisopropylamino-1,2-propanediol acetonide in methanol (1liter) and water (325 ml). The mixture was stirred under reflux for 90min. The cooled mixture was filtered and the filtrate was concentratedin vacuo. The residue was evaporated several times from benzene-EtOHmixtures to remove the last traces of water. The resulting solid wastriturated with 2.5 l ether to give the diol, mp 67°-70°. Concentrationof the ether furnished additional diol, mp 64°-66°. Crystallization fromethyl acetate-hexane furnished the analytically pure material, mp73°-74°; [α]_(D) ²⁵ -15.94° (c, 1.0, H₂ O).

Anal. Calcd. for C₇ H₁₇ NO₄ S: C, 39.79; H, 8.11; N, 6.63; S, 15.18.Found: C, 39.83; H, 8.40; N, 6.66; S, 14.96.

EXAMPLE 5 (S)-1-Mesyloxy-2-hydroxy-3-N-mesylisopropylaminopropane.

19.1 g (90.5 mmol) N-mesyl-1,2-dihydroxy-3-isopropylaminopropane wasdissolved in 150 ml anhydrous pyridine under argon and cooled to -45°.7.0 ml (90.4 mmol) mesyl chloride was added dropwise over 5 minutes andthe mixture was stirred at -45° for 5 hrs. The cold mixture was thendiluted with 100 ml H₂ O followed by 200 ml 6 N HCl and extracted withEtOAc (3x). The EtOAc layers were washed in turn with 3 N HCl (1×250ml), brine (2x) and NaHCO₃ solution (1x). The combined extracts werecombined and evaporated to give the O,N-dimesylate as an oil whichsolidified on standing.

This material was contaminated with 2% trimesylate. A small sample waspurified in the following way: 500 mg was dissolved in 10 ml water andfiltered free of any undissolved trimesylate. The filtrate was extractedwith ether (2x) and with EtOAc (2x). The EtOAc layers were combined,dried (Na₂ SO₄), and evaporated. Crystallization of the residue fromether furnished the analytical sample, mp 51°-52°, [α]_(D) ²⁵ -1.21° (c,1.0, H₂ O).

Anal. Calcd. for C₈ H₁₉ NO₆ S₂ : C, 33.21; H, 6.59; N, 4.84; S, 22.16.Found: C, 33.33; H, 6.13; N, 4.76; S, 22.18.

EXAMPLE 6 (S)-1-Tosyloxy-2-hydroxy-3-N-mesylisopropylaminopropane.

A stirred solution of 63.3 g (0.3 mol) diol of Example 4 in 450 mlpyridine was cooled to -20° and 85.5 g tosyl chloride (0.45 mol) wasadded. The reaction mixture was stirred in an ice-water bath for 2 hrwhereupon 5-10 g ice was added to the mixture and the stirring wascontinued for an additional 15 minutes before it was poured into amixture of ice (1 kg) and concentrated HCl (500 ml) and extracted withCH₂ Cl₂ (1×1 l; 2×500 ml). The extracts were washed in turn with brine(1×500 ml) and 5% NaHCO₃ solution. The combined, dried (Na₂ SO₄)extracts were concentrated in vacuo to give the mono-tosylate as an oil.A small portion was purified for analysis by chromatography; [α]_(D) ²⁵+5.65° (c, 1.0, CHCl₃).

Anal. Calcd. for C₁₄ H₂₃ NO₆ S₂ : C, 46.01; H, 6.34; N, 3.83; S, 17.55.Found: C, 46.11; H, 6.53; N, 3.67; S, 16.97.

EXAMPLE 7 (2S)-1-Chloro-2-hydroxy-3-N-mesylisopropylaminopropane.

A mixture of 10.55 g (50 mmol) of diol of Example 4, 9.0 g (75 mmol) oftrimethylorthoacetate and 0.4 g (3.27 mmol) of benzoic acid was heatedwith stirring at 80° for 45 min. The viscous reaction mixture was cooledand partioned between CH₂ Cl₂ and saturated NaHCO₃ solution. The CH₂ Cl₂layer was dried (Na₂ SO₄) and evaporated to give 14.5 g of the crudeorthoacetate which was then dissolved in 75 ml dry CH₂ Cl₂ and treatedwith 12 ml (95 mmol) trimethylchlorosilane and heated at reflux for 60minutes. The solvent was removed in vacuo and the residue was dissolvedin 100 ml 0.3 N methanolic HCl and the mixture was left at ambienttemperature for 65 hrs. The solvent was removed under reduced pressureand the residual oil was chromatographed over silica gel (50 g) to givethe pure chlorohydrin as an oil, [α ]_(D) ²⁵ -12.7° (c, 1.0 MeOH).

Anal. Calcd. for C₇ H₁₆ ClNO₃ S: C, 36.60; H, 7.02; N, 6.10; Cl, 15.43;S, 13.96. Found: C, 36.38; H, 7.32; N, 5.98; Cl, 15.15; S, 13.53.

EXAMPLE 8 (2S)-3-Benzyloxypropandiol, 1,2-acetonide.

44 g of a 50% dispersion of NaH in oil was placed in a flask and washedwith dry hexane. To the flask was then charged a solution of 120 gbenzyl chloride in 1500 ml dry DMF. To this stirred mixture, a solutionof (2S)-glycerol-2,3-acetonide (120 g) in dry DMF (500 ml) was addedover 45 min at ambient temperature (initial reaction temperature was 17°and it rose to a maximum of 27° during the addition). Stirring wascontinued for 1 hr after the addition was completed and then MeOH (30ml) was added dropwise to destroy excess hydride. The system was thenequipped for distillation and the DMF was distilled off in vacuo(55°-60°; water aspirator). The residue was diluted with brine (2liters) and extracted with CH₂ Cl₂ (3×1 liter). The organic layers werewashed in turn with brine and then were combined, dried (MgSO₄) andevaporated to give an oil.

The oil was distilled in vacuo to give the benzyl ether (bp 78°-80°,0.05 mm).

EXAMPLE 9 (2R)-3-Benzyloxy-1,2-propanediol.

A suspension of 75 ml Dowex 50-8x (H⁺ form) in a stirred solution of254.6 g (1.15 mol) (2S)-3-benzyloxypropanediol, acetonide in 800 ml MeOHand 200 ml H₂ O was heated at reflux for 1 hr. The resin was filteredoff and the filtrate was concentrated in vacuo. The diol was freed fromresidual H₂ O by evaporating it several times from ethanol-benzenemixtures to give it as a colorless oil.

EXAMPLE 102(R,S),4(S)-2-Methoxy-2-methyl-4[(benzyloxy)methyl)]-1,3-dioxolane

A stirred solution of 207 g (1.13 mol) (2R)-3-benzyloxy-1,2-propanediolin 200 g (1.66 mol) trimethylorthoacetate containing 3 g (0.024 mol) washeated in an oil bath at 75° in a flask fitted for downwarddistillation. Methanol thus was removed from the reaction as it wasformed. After 40 min the reaction mixture was cooled and partitionedbetween CH₂ Cl₂ (800 ml) and 1 N NaOH solution (200 ml). The aqueousphase was extracted further with CH₂ Cl₂ (2×250 ml) and then the organiclayers were washed in turn with 200 ml 0.5 N NaOH solution. The combinedCH₂ Cl₂ layers were dried (Na₂ SO₄) and concentrated to dryness to givethe cyclic orthoacetate as a colorless oil.

Analysis of its nmr spectrum showed it to be a 4:3 mixture ofdiastereoisomers. A small sample was distilled (˜165°/0.1 mm) to givethe analytical sample [α]_(D) ²⁵ +7.4° (c, 1.0, MeOH).

Anal. Calcd. for C₁₃ H₁₈ O₄ : C, 65.53; H, 7.61. Found: C, 65.84; H,7.76.

EXAMPLE 11 (2S)-3-Benzyloxy-2-acetoxy-1-chloropropane

155 ml. (1.21 mol) trimethylchlorosilane was added to a solution of 270g (1.13 mol) crude cyclic orthoacetate in 600 ml dry CH₂ Cl₂ and themixture was heated at reflux in an inert atmosphere for 30 minutes. Thesolvent and excess reagent were removed under reduced pressure to givethe crude chloroacetate as a mobile oil. This material was used withoutfurther purification.

A small portion was distilled (˜165°/0.1 mm) to give the analyticalsample [α]_(D) ²⁵ +10.8° (c, 1.0, MeOH).

Anal. Calcd. for C₁₂ H₁₅ ClO₃ : C, 59.39; H, 6.23; Cl, 14.61. Found: C,59.50; H, 6.31; Cl, 14.35.

EXAMPLE 12 (2S)-1,2-Epoxy-3-benzyloxypropane.

A chilled solution of 115 g NaOH (2.875 mol) in 600 ml H₂ O was addeddropwise over 20 minutes to a cooled solution of the crude chloroacetateof Example 11 (theory ˜1.13 mol) in MeOH (800 mol) with stirring. Thereaction temperature was maintained at <12° during the addition. Afterstirring an additional 1 hr at ˜12°, the reaction was cooled to 5° andthen neutralized to pH 7.0 using dilute H₂ SO₄. The reaction was thenconcentrated in vacuo (bath temp. ˜25°) to remove MeOH and then it wasdiluted with 250 ml brine and extracted with CH₂ Cl₂ (1×750 ml; 1×400ml). The CH₂ Cl₂ extracts were combined, dried (Na₂ SO₄) and evaporatedto dryness. The resulting oil was distilled in vacuo to give theepoxide, bp 84°-86°/0.45 mm; [α]_(D) ²⁵ - 10.64° (c, 1.0, MeOH).

Anal. Calcd. for C₁₀ H₁₂ O₂ : C, 73.15; H, 7.27. Found: C, 73.21; H,7.51.

EXAMPLE 13(S)-1-(4-Benzyloxyphenoxy)-2-hydroxy-3-N-mesylisopropylaminopropaneMethod A-Condensation of the product of Example 6 with benzyloxyphenol

A solution of (S)-1-tosyloxy-2-hydroxy-3-N-mesylisopropylaminopropane(103 g, 0.282 mol) and p-benzyloxyphenol (79 g, 0.395 mol) in 500 mlDMSO was treated with 93.7 ml 4 N NaOH (0.375 mol) and stirred at 100°under argon for 21/2 hr. The solution was cooled and 300 ml 1 N NaOH wasadded slowly to the vigorously stirred solution followed by 600 ml H₂ O.The resulting solid was removed by filtration and was washed with water.After partial air drying, the solid was dissolved in CH₂ Cl₂ and thesolution was dried over MgSO₄. The decolorized (charcoal) solution wasevaporated to dryness and the resulting solid residue was trituratedwith hot ether (1.5 liters). Filtration of the colorless solid gave theend product, mp 91°-93°.

Recrystallization from CH₂ Cl₂ -ether gave the pure sample, mp 94°-95°;[α]_(D) ²⁵ -0.93° (c, 1.0, CHCl₃).

Anal. Calcd. for C₂₀ H₂₇ NO₅ S: C, 61.04; H, 6.91; N, 3.56. Found: C,61.04; H, 6.90; N, 3.43.

EXAMPLE 14 Method B--Condensation of the product of Example 7 withbenzyloxyphenol to give product of Example 13

A solution of 8.3 g (36.1 mmol) of(2S)-1-chloro-2-hydroxy-3-N-mesylisopropylaminopropane and 8.69 g (43.4mmol) of p-benzyloxyphenol in 75 ml DMSO was treated with 10.86 ml 4 NNaOH (43.4 mmol) and the mixture was heated at 100° for 5 hours. To thecooled reaction mixture 40 ml 1 N NaOH and 60 ml H₂ O were added withstirring and the resulting solid was collected by filtration and waswashed well with H₂ O. The dried crude product was recrystallized fromEtOAc-hexane to yield the end product as white needles, mp 93°-94°.

EXAMPLE 15(S)-1-(4-Hydroxyphenoxy)-2-hydroxy-3-N-mesylisopropylaminopropane

A slurry of 10% Pd/C in 30 ml EtOAc was added to a solution of 38.4 g(S)-1-(4-benzyloxyphenoxy)-2-hydroxy-3-N-mesylisopropylaminopropane in850 ml MeOH and the mixture was hydrogenated (760 mm; 20°). Within 1 hrthe uptake of H₂ stopped (total 2.6 liters) and the catalyst was removedby filtration through Celite. The filtrate was concentrated underreduced pressure and the residue was crystallized from CH₂ Cl₂ -ether togive the phenol, mp 91°-93°. Recrystallization of a sample fromEtOAc-hexane gave the analytically pure material, mp 92°-94°; [α]-1.93°(c, 1.0, CHCl₃).

Anal. Calcd. for C₁₃ H₂₁ NO₅ S: C, 51.47; H, 6.98; N, 4.62; S, 10.57.Found: C, 51.38; H, 6.89; N, 4.58; S, 10.46.

EXAMPLE 16 (S)-4-(2-Hydroxy-3-N-mesylisopropylaminopropoxy)phenoxyaceticacid

To a stirred solution of a 24 g (0.08 mol) of(S)-1-(4-hydroxyphenoxy)-2-hydroxy-3-N-mesylisopropylaminopropane in 240ml of absolute ethanol (argon atmosphere) was added at room temperature9.04 g (0.08 mol) of potassium t-butoxide. After stirring for 15 min,13.36 g (0.08 mol) of ethyl bromoacetate was added and the reactionmixture was heated under reflux overnight. It was then cooled andconcentrated in vacuo. The residue was acidified to pH 2 with 1 N HCl,and was then dissolved in EtOAc. The organic layer was then washed twicewith 1 N NaOH, once with H₂ O and dried (Na₂ SO₄). Evaporation of thesolvent gave the crude ester as an oil which solidified on standing.

To a solution of 29 g (0.75 mol) of crude ester in 290 ml of MeOH, wasadded 40 ml of 4 N NaOH solution. The reaction mixture was refluxed forten minutes and then allowed to cool to room temperature for one hour.Most of the solvent was evaporated in vacuo and the residue treated with50 ml of 6 N HCl solution. The resulting solid was collected byfiltration and crystallized twice from acetone-hexane to yield the endproduct, mp 128°-129°. Crystallization from acetone-hexane gave theanalytical sample, mp 129°-130°; [α]_(D) ²⁵ +1.5° (c, 1.0, CH₃ OH).

Anal. Calcd. for C₁₅ H₂₃ NO₇ S: C, 49.85; H, 6.41; S, 8.87; N, 3.87.Found: C, 49.84; H, 6.58; S, 8.83; N, 3.80.

EXAMPLE 17 1-(2-Chloroethyl)-4-phenylpiperazine

To a stirred solution of N-phenylpiperazine (95% pure; 300 g; 1.85 mol)in 1 l MeOH previously chilled to -20° was added in one portion achilled solution of ethylene oxide (150 ml) in MeOH (250 ml). Themixture was stirred in an ice-water bath overnight. After the solventand excess reagent were removed in vacuo, the residue was taken up intoluene and re-evaporated to remove residual MeOH.

The crude 1-(2-hydroxyethyl)-4-phenylpiperazine (˜400 g) thus obtainedwas dissolved in dry CH₂ Cl₂ (3.5 l) containing triethylamine (400 ml;2.9 mol) and the solution was cooled to ˜10. A solution of 250 g (2.18mol) mesyl chloride in 300 ml CH₂ Cl₂ was added to the stirred mixtureover 30 min and then the reaction was allowed to warm up to room temp.It was then stirred at ambient temperature until the in situ conversionof the intermediate mesylate to the chloro compound was completed (16-40hrs). Water (1 l) was added and the layers separated. The aqueous layerwas washed with CH₂ Cl₂ (1×300 ml) and then the combined CH₂ Cl₂ phaseswere dried (K₂ CO₃) and evaporated. The residue was triturated with hothexane (1×1 l; 4×200 ml) and the combined extracts were decolorized(charcoal) and then cooled to 0°- 5°. Filtration of the resultingcolorless crystals afforded 1-(2-chloroethyl)-4-phenylpiperazine, mp56°-58°. Concentration of the mother liquor to ˜400 ml furnishedadditional product, mp 55°-57°.

Recrystallization of a small sample from hexane furnished theanalytically pure material, mp 59°-60°.

Anal. Calcd. for C₁₂ H₁₇ ClN₂ : C, 64.13; H, 7.62; N, 12.47; Cl, 15.78.Found: C, 64.30; H, 7.61; N, 12.41; Cl, 15.57.

EXAMPLE 18 1-(2-Chloroethyl)-4-(2-methoxyphenyl)-piperazine

In a procedure, analogous to the one described above, 50 mmol ofN-(2-methoxyphenyl)piperazine was converted to1-(2-chloroethyl)-4-(2-methoxyphenyl)-piperazine, mp 36.5°-37°.

Anal. Calcd. for C₁₃ H₁₉ ClN₂ O: C, 61.29; H, 7.52; N, 10.99; Cl, 13.92.Found: C, 61.22; H, 7.52; N, 11.02; Cl, 13.93.

EXAMPLE 19 1-[2-(4-Benzyloxyphenoxy)ethyl]-4-phenylpiperazine

26.25 ml 4 N NaOH (0.105 mol) was added to a stirred mixture of 21.0 g(0.105 mol) 4-benzyloxyphenol and 22.5 g (0.1 mol) of1-(2-chloroethyl)-4-phenylpiperazine in 250 ml DMSO and the reaction washeated at 60° for 60 min. After cooling, the stirred mixture was dilutedwith 50 ml 1 N NaOH solution and the resulting crystalline precipitatewas collected by filtration and washed with water to give, after dryingin vacuo, essentially pure end product, mp 116°-119°.

A small sample was recrystallized from ethyl acetate for analysis, mp120°-121°.

Anal. Calcd. for C₂₅ H₂₈ N₂ O₂ : C, 77.29; H, 7.26; N, 7.21. Found: C,77.19; H, 7.08; N, 7.12.

EXAMPLE 20 1-[2-(4-Hydroxyphenoxy)ethyl]-4-phenylpiperazine. MethodA--By HCl cleavage of the benzyl ether of Example 19

35.75 g of 1-[2-(-4-benzyloxyphenoxy)ethyl]-4-phenylpiperazinephenylpiperazine was added rapidly with stirring to 75 ml concentratedHCl and the mixture was heated on a steam bath for 15 minutes. Duringthis time starting material dissolved and then a white crystalline solidbegan to form and eventually the reaction mixture almost solidified. Thereaction was cooled to ˜5° and then was diluted with 100 ml EtOH. Thesolids were filtered off and were washed in EtOH and ether to give1-[2-(4-hydroxyphenoxy)ethyl]-4-phenylpiperazine as itsmonohydrochloride. The salt was dissolved in 150 ml hot MeOH and 50 mlH₂ O and was then treated with 25 ml triethylamine. Water was then addedto the refluxing mixture just to the cloud point whereupon the productbegan to crystallize from solution. The mixture was chilled and thesolids were collected by filtration to give the end product, mp142°-143°.

The analytically pure material, 143°-144°, was obtained byrecrystallization from EtOAc.

Anal. Calcd. for C₁₈ H₂₂ N₂ O: C, 72.46; H, 7.43; N, 9.39. Found: C,72.32; H, 7.23; N, 9.25.

EXAMPLE 21 Method B--Hydrogenolysis of benzyl group in the product ofExample 19 to produce the end product of Example 20

1-(4-Benzyloxyphenoxy)-4-phenylpiperazine (211.2 g; 0.544 mol) in 1 lHOAc was hydrogenolyzed over 20 g of 10% Pd/C (21°; 1 atmos). Theabsorption of H₂ essentially stopped after 3 hr (total uptake 15.1 l).The catalyst was filtered off through Celite and the filtrate wasconcentrated to dryness in vacuo. The crude material was dissolved in750 ml hot MeOH and 250 ml warm (˜70°) H₂ O was added followed by 120 mlof triethylamine. H₂ O was then added to the cloud point whereupon theproduct started to crystallize rapidly from solution. The mixture wascooled to ˜5° and the crystalline material was removed by filtration andwashed with MeOH-H₂ O (1:1) to give, the end product, mp 143°-144°.

EXAMPLE 22(S)-1-[2-(4-(2-Hydroxy-3-N-mesylisopropylaminopropoxy)phenoxy)ethyl]-4-phenylpiperazine.Method A--Condensation of the compound of Example 15 with1-(2-chloroethyl)-4-phenylpiperazine.

11.0 ml 4 N NaOH solution (44 mmol) was added to a stirred solution of13.3 g (43.9 mmol)(S)-1-(4-hydroxyphenoxy)-2-hydroxy-3-N-mesylisopropylaminopropane and10.0 g (44.4 mmol) 1-(2-chloroethyl)-4-phenylpiperazine in 100 ml DMSO.The mixture was heated at 60° under argon and then was cooled anddiluted with 200 ml water. The resulting solid was collected byfiltration, was washed with water and then was dissolved in CH₂ Cl₂. TheCH₂ Cl₂ solution was washed with 5% Na₂ CO₃ solution and then was dried(K₂ CO₃) before evaporation in vacuo to a white solid. Crystallizationof the product from EtOAc furnished the end product in two crops: mp104°-106°; mp 103°-105°.

A small sample of the second crop was recrystallized from EtOAc to givethe analytically pure material, mp 104°-106°; [α]_(D) ²⁵ -0.5° (c, 1%,CHCl₃).

Anal. Calcd. for C₂₅ H₃₇ N₃ O₅ S: C, 61.08; H, 7.59; N, 8.55; S, 6.52.Found: C. 61.14; H, 7.74; N, 8.40; S, 6.41.

EXAMPLE 23 Method B--Condensation of the product of Example 6 and1-[2-(4-hydroxyphenoxy)-ethyl]-4-phenylpiperazine

300 ml 4 N NaOH (1.2 mol) was added to a stirred mixture of 362 (0.99mol) (S)-1-tosyloxy-2-hydroxy-3-N-mesylisopropylaminopropane and 298.4 g(1 mol) 1-[2-(4-hydroxyphenol)ethyl]-4-phenylpiperazine in 2.4 l DMSOand the reaction was heated at 95°-100° for 12 hrs. The cooled mixturewas diluted with 1 l 1 N NaOH and 7 l H₂ O and was extracted usingbenzene (1×12 l; 1×2). The organic extracts were washed in turn withwater (2×2 l) and then were dried (K₂ CO₃) and evaporated to dryness. Asolution of the residue in hot EtOAc was charcoaled and, afterconcentration to ˜2.5 l was stored at 0°-5° overnight to give the endproduct to Example 22. The mother liquors were concentrated to ˜500 mland diluted with 500 ml hexane which yielded additional product. The twocrops were combined and recrystallized from acetone-hexane to giveessentially pure material, mp 95°-100°.

EXAMPLE 24 (S)-1-(4-Benzyloxyphenoxy)-3-benzyloxy-2-propanol

4.5 g (0.04 mol) KOC(CH₃)₃ was added to a solution of 66.3 g (0.404 mol)(S)-1,2-epoxy-3-benzyloxypropane and 97.0 g (0.484 mol)p-benzyloxyphenol in 240 ml MeOH and the mixture was heated overnight atreflux. The reaction was cooled and then was diluted with the slowaddition of 1 l 1 N NaOH. The resulting solids were recovered byfiltration and were washed with 0.5 N NaOH and with water and air dried.The material was dissolved in 800 ml CH₂ Cl₂ and the solution was washedwith H₂ O (3×150 ml). The water layers were backwashed in turn with 200ml CH₂ Cl₂ and concentration of the combined, dried (Na₂ SO₄) CH₂ Cl₂layers gave essentially pure product.

A small sample was crystallized from CH₂ Cl₂ -hexane (2x) to give theanalytically pure material, mp 62°-63.5°; [α]_(D) ²⁵ +4.4° (c, 1.0,MeOH).

Anal. Calcd. for C₂₃ H₂₄ O₄ : C, 75.80; H, 6.64. Found: C, 75.82; H,6.60.

EXAMPLE 25 (S)-3-(4-Hydroxyphenoxy)-1,2-propanediol

A solution of 138 g (0.378 mol)(S)-1-(4-benzyloxyphenoxy)-3-benzyloxy-2-propanol in 1.7 liters HOAccontaining 36 ml concentrated HCl was hydrogenated over 14 g 10% Pd/C atnormal temp and pressure. The uptake of hydrogen essentially stoppedafter 70 min (˜17.7 liters H₂ used). The catalyst was removed byfiltration through Celite and concentration of the filtrate to drynessgave an oil which was then dissolved in 800 ml 0.5 N methanolic HCl andleft at room temp overnight to hydrolyze the acetates which had beenformed during the hydrogenation. The reaction was concentrated todryness and the residue was evaporated several times from CH₂ Cl₂ toeliminate residual HCl. Trituration of the resulting solid with etherfurnished the essentially pure end product.

0.5 g was crystallized from MeOH-CHCl₃ to give the analytically purematerial, mp 149.5°-151°; [α]_(D) ²⁵ +8.01° (c, 1.0, MeOH).

Anal. Calcd. for C₉ H₁₂ O₄ : C, 58.69; H, 6.57. Found: C, 58.43; H,6.73.

EXAMPLE 26 (R)-1-(4-Hydroxyphenoxy)-3-chloro-2-propanol.

A stirred mixture of 62.15 g (0.337 mol)(S)-3-(4-hydroxyphenoxy)-1,2-propanediol (0.025 mol) and benzoic acid in60 g trimethylorthoacetate was heated in an oil bath at 80°. Methanolwas distilled from the system as it was formed. After 30 minutes thereaction mixture was cooled and partitioned between 750 ml benzene and250 ml saturated NaHCO₃ solution. The benzene layer was washed withanother 250 ml portion of NaHCO₃ solution and then the aqueous layerswere backwashed with 250 ml benzene. The combined organic contracts weredried (Na₂ SO₄) and evaporated in vacuo to give the cyclic orthoacetateas an oil. The total crude material was then dissolved in 400 ml dry CH₂Cl₂ and treated with 65 ml trimethylchlorosilane. After being heated atreflux for 30 minutes, the solution was concentrated to dryness underreduced pressure. The residue was evaporated from toluene to removeexcess reagent to give crude (R)-3-(4-hydroxyphenoxy)-2-acetoxy-1-chloropropane as an oil. The acetate was hydrolyzed by heating asolution of the crude product in 250 ml 0.5 N methanolic HCl at 55° for30 min. The solvent was concentrated in vacuo and the residue wasdiluted with water and was extracted with EtOAc to give the crudechlorohydrin. The crude material was dissolved in 120 ml CH₂ Cl₂ andplaced on a column of silica gel (800 g made up in CH₂ Cl₂). Threeliters of CH₂ Cl₂ eluent were collected and discarded and the productwas eluted using 3 liters of CH₂ Cl₂ EtOAc (1:1) to give afterevaporation of the solvent, the end product as an oil.

EXAMPLE 27(S)-1,2-Epoxy-3-[4-(2-(4-phenyl-1-piperazinyl)ethoxy)phenoxy]propane

29.5 ml 4 N NaOH (0.118 mol) was added at a rapid dropwise rate to astirring solution of 12 g (59 mmol)(R)-1-(4-hydroxyphenoxy)-3-chloro-2-propanol in 210 ml DMSO. During theaddition the temp of the reaction was maintained <25° by means of acooling bath. After the reaction had stirred at ˜20° for 10 min, 12.6 g(56.3 mmol) 1-(2-chloroethyl)-4-phenylpiperazine was added in oneportion and the mixture was heated at 40° for 21/2 hr (after 20 min, asolid material began to crystallize from solution). The mixture wascooled and diluted with 30 ml H₂ O. The crystalline precipitate wascollected by filtration and was washed with 100 ml DMSO-H₂ O (3:1) andwith water. After air drying, the solids were dissolved in benzene (600ml) and washed with H₂ O (3×150 ml). The aqueous layers were backwashedwith benzene (1×200 ml). The combined benzene extracts were dried (K₂CO₃) and evaporated to give the end product as a white solid.

A small sample from a previous run was filtered through a short silicagel column and crystallized from acetone to give the analytically purematerial, mp 118°-119.5°; [α]_(D) ²⁵ -10.1° (c, 1.0, MeOH).

Anal. Calcd. for C₂₁ H₂₆ N₂ O₃ : C, 71.16; H, 7.39; N, 7.90. Found: C,71.19; H, 7.28; N, 7.92.

EXAMPLE 28 (S)-1-(4-Benzyloxyphenoxy)-2-hydroxy-3-isopropylaminopropane

Under a flow of argon 318 ml of 70% Red-al solution was added over 15min to a stirred solution of 86.5 g of(S)-1-(4-benzyloxyphenoxy)-2-hydroxy-3-N-mesylisopropylaminopropane in800 ml dry benzene. After the addition was complete, the mixture wasrefluxed with stirring for 3 hours. The reaction was cooled using anice-water bath and 30 ml 1 N NaOH was added dropwise to destroy excesshydride, followed by 800 ml 2 N NaOH. The phases were separated and theorganic layer was washed in 1 N NaOH and with water. The aqueous phaseand washings were back extracted with benzene (1×500 ml). The combinedbenzene extracts were dried (anhydr. K₂ CO₃) and evaporated to give asolid. The crude material was dissolved in 1500 ml ether and thesolution was concentrated to ˜750 ml. The resulting crystalline aminewas filtered off to give pure material, mp 93°-95°; [α]_(D) ²⁵ -6.26°(c, 1.0, CHCl₃).

Concentration of the mother liquors gave two additional crops ofproduct: Crop #2, mp, 91°-93° and Crop #3, mp, 89°-93°.

Anal. Calcd. for C₁₉ H₂₅ NO₃ : C, 72.35; H, 7.99; N, 4.44. Found: C,72.38; H, 8.16; N, 4.43.

EXAMPLE 29 (S)-1-(4-Hydroxyphenoxy)-2-hydroxy-3-isopropylaminopropaneMethod A - From(S)-1-(4-benzyloxyphenoxy)-2-hydroxy-3-isopropylaminopropane

A slurry of 5.4 g 10% Pd/C in 50 ml benzene was added to a solution of54.1 g of the amine in 600 ml MeOH. The attempted hydrogenolysis of thismixture resulted in very slow uptake of hydrogen due to catalystpoisoning by minor sulfur containing impurities in the amine. Thepoisoned catalyst was replaced by 5.4 g fresh Pd/C and then 4.2 litersof H₂ was taken up within 40 minutes. The catalyst was removed byfiltration and the filtrate was concentrated in vacuo. Crystallizationof the residue from acetone furnished the phenol amine, mp 125°-127°;[α]₂₅ ^(D) -22.1° (C, 1.0, 0.1 N HCl). The analytically pure sample, mp125°-127°, was crystallized from acetone.

Anal. Calcd. for C₁₂ H₁₉ NO₃ : C, 63.97; H, 8.50; N, 6.22. Found: C,63.81; H, 8.68; N, 6.40.

EXAMPLE 30 Method B - From (R)-1-(4-Hydroxyphenoxy)-3-chloro-2-propanolto produce the end product of Example 29

A solution of 50 g (0.247 mol)(R)-1-(4-hydroxyphenoxy-3-chloro-2-propanol in 300 ml methanolcontaining 100 ml isopropylamine was refluxed overnight. The solvent wasremoved in vacuo and the residue was evaporated twice from methanol toeliminate remaining isopropylamine. The crude was dissolved in 250 ml 1N HCl and the solution was extracted with ether (2x) to remove non-basicimpurities. The aqueous layer was chilled in an ice-water bath and wastreated with 13.5 g Na₂ CO₃ (0.255 equiv.). After 5 min the phenolaminebegan to crystallize from solution. The mixture was stirred in theice-water bath for 30 min and then was stored at 0° for 1 hr. Theproduct was collected by filtration and was washed with water to givethe phenolamine, mp 124°-126°. 60 g of NaCl was added to the combinedfiltrate and washings and the resulting solution was extracted with6×200 ml portions of EtOAc. The extracts were dried (K₂ CO₃) andevaporated to give an additional amount of phenolamine.

Crystallization of the total product from acetone (charcoal) furnishedpure phenol mp 124°-126°; [α]_(D) ²⁵ -22.0° (c, 1.0, 0.1 N HCl).

EXAMPLE 31(S)-1-[2-(4-(2-Hydroxy-3-(1-methylethyl)amino)propoxy)-phenoxy)ethyl]-4-phenylpiperazineand its bis-maleate salt Method A

A solution of 15.35 g (42.5 mmol) of(S)-4-(2-hydroxy-3-N-mesylisopropylaminopropoxy)phenoxyacetic acid in170 ml of dry tetrahydrofuran was cooled (0°). To this solution (argonatmosphere) was added 5.16 g (51 mmol) of triethylamine followed by thedropwise addition of 4.61 g (42.5 mmol) of ethyl chloroformate. Thereaction mixture was allowed to stir at 0° for one hr and was thenfiltered from the resulting precipitate. The filtrate was then treatedwith 10.35 g (63.8 mmol) of N-phenylpiperazine and the reaction mixturewas allowed to stir under argon for 90 min at room temp. The solvent wasthen evaporated in vacuo and the residue was dissolved in methylenechloride and treated with 100 ml of 5 N HCl solution. The aqueous layerwas extracted twice with methylene chloride. The organic layers werethen washed twice with 5 N HCl solution and once with 5% NaHCO₃solution. The combined organic layers were dried (Na₂ SO₄) andevaporated to give an oil which was dissolved in benzene andchromatographed on silica gel (400 g). The column was eluted withbenzene-ethyl acetate mixtures, the product being eluted withbenzene-ethyl acetate (3:1) and pure ethyl acetate. Evaporation of thesesolvent fractions yielded the intermediate amide1-[(S)-4-(2-hydroxy-3-N-mesylisopropylaminopropoxy)phenoxyacetyl]-4-phenylpiperazineas an oil.

To a portion of this material (18 g, 35.7 mmol) in 600 ml of dry benzenewas added dropwise (over 25 min) 113 ml of 70% sodiumbis(2-methoxyethoxy)-aluminum hydride in benzene (Red-al). The reactionmixture was heated under reflux for 30 min and was then cooled andtreated dropwise with 120 ml of 1 N NaOH solution followed by 1 l of H₂O. The resulting mixture was extracted twice with 1500 ml of benzene andthe organic layers were washed twice with water. The dried (Na₂ SO₄)organic layers were concentrated in vacuo to give a colorless solid, mp88°-95°. Crystallization from methylene chloride-ether gave the endproduct, mp 99°-102°. Further crystallization from the same solventsystem gave the analytical sample, mp 102°-104°; [α]_(D) ²⁵ -0.3° (c,1.0, CH₃ OH).

Anal. Calcd. for C₂₄ H₃₅ N₃ O₃ : C, 69.70; H, 8.53; N, 10.16. Found: C,69.73; H, 8.65; N, 10.00.

Method B - From(S)-1-(4-hydroxyphenoxy)-2-hydroxy-3-isopropylaminopropane

18.75 ml 4 N NaOH (75 mmol) was added to a solution of 16.0 g (71.4mmol) 1-(2-chloroethyl)-4-phenylpiperazine and 16.8 g (75 mmol)1-(4-hydroxyphenoxy)-2-hydroxy-3-isopropylaminopropane, 150 ml DMSO andthe mixture was stirred at 60° for 3 hours. 100 ml 1 N NaOH was addedslowly to the cooled solution followed by 100 ml H₂ O and the resultingsolid was recovered by filtration and was washed well with water. Thecrude solid was dissolved in CH₂ Cl₂ (600 ml) and the solution was dried(K₂ CO₃) and evaporated to give the crude product. The material wasdissolved in 400 ml hot EtOAc and diluted with 400 ml hexane and wasstored overnight 0°-5°. The resulting solid was collected by filtrationand was washed with 200 ml EtOAc-hexane (1:1) to give the end product,mp 102° -104°.

23.3 g (56.4 mmol) of the amine was added to a hot solution of maleicacid (13.34 g; 115 mmol) in 300 ml MeOH. Hot EtOAc was added portionwiseto the refluxing solution such that the volume remained at ˜500 ml untilthe salt began to crystallize from the solution. The mixture was cooledand the bis-maleate salt, mp 151°-153° was recovered by filtration.

Recrystallization from the same solvent raised the melting point to153°-154°; [α]_(D) ²⁵ -10.8° (c, 1.0, MeOH).

Anal. Calcd. for C₂₄ H₃₅ N₃ O₃.2C₄ H₄ O₄ : C, 59.52; H, 6.71; N, 6.51.Found: C, 59.59; H, 6.55; N, 6.56.

Method C - From(S)-1-[2-(4-(2,3-epoxypropoxy)phenoxy)ethyl]-4-phenylpiperazine

A solution of 7.8 g (22 mmol)(S)-1-[2-(4-(2,3-epoxypropoxy)phenoxy)ethyl]-4-phenylpiperazine in 80 mlMeOH containing 20 ml isopropylamine was heated at 75° for 2 hr. Thesolvent was removed in vacuo and the residue was dissolved in 100 mlMeOH containing 5.1 g (44 mmol) maleic acid. The slightly hazy solutionwas filtered through Celite and then was concentrated to ˜60 ml,whereupon hot EtOAc was added until crystallization began. The resultingsalt was recovered by filtration and washed with EtOAc to give thebis-maleate salt, mp 150°-151.5°.

Method D - From(S)-1-[2-(4-(2-hydroxy-3-N-mesylisopropylaminopropoxy)phenyl)ethyl]-4-phenylpiperazine

In an inert atmosphere, 82 ml Red-al (70% solution in benzene) was addeddropwise to a stirred solution of 19.2 g (39 mmol)(S)-1-[2-(4-(2-hydroxy-3-N-mesylisopropylaminopropoxy)phenoxy)-ethyl]-4-phenylpiperazinein 100 ml benzene. After heating at reflux for 31/2 hr, the mixture wascooled in an ice-water bath and excess reagent was destroyed by thecautious dropwise addition of H₂ O. The mixture was then diluted with 2N NaOH and CH₂ Cl₂ which resulted in the precipitation of some insolubleinorganic solids. The solids were removed by filtration through Celiteand the filtrate was extracted with CH₂ Cl₂ (3x). The organic layerswere washed in turn with 2 N NaOH solution (2x) and brine (1x) and thenwere combined, dried (K₂ CO₃) and evaporated to give the amine as acrystalline solid. A solution of the solids in 125 ml hot EtOAc wasdiluted with warm hexane (125 ml) and the product was allowed tocrystallize slowly from solution. Filtration yield the amine, mp100°-104°.

13.9 g (33.6 mmol) of the pure amine was added to a hot solution of 7.96g (68.6 mmol) maleic acid in 100 ml MeOH. Hot EtOAc was added inportions to the refluxing solution until crystallization of the saltbegan. After cooling, the mixture was filtered to give the bis-maleatesalt, mp 152°-154°.

EXAMPLE 32(S)-1-[2-(4-(2-Hydroxy-3-tert-butylaminopropoxy)-phenoxy)ethyl]-4-phenylpiperazineand its bis-maleate salt

A solution of 7.8 g (22 mmol) of(S)-1-[2-(4-(2,3-epoxypropoxy)phenoxy)ethyl]-4-phenylpiperazine in 80 mlMeOH containing 25 ml tert-butylamine. The solvent was removed underreduced pressure to give an oily residue which crystallized on standing.The crude amine was dissolved in 60 ml hot MeOH containing 5.1 g (44mmol) maleic acid. Almost immediately a crystalline solid began to form.The mixture was cooled and filtered to yield the bis-maleate, mp180°-182°. A second crop, mp 172°-175° recovered from the motherliquors, was recrystallized from MeOH to give the salt, mp 179°-181°.

The first crop was recrystallized from MeOH to give the analyticallypure salt, mp 182°-184°; [α]_(D) ²⁵ -6.03° (c, 0.58, H₂ O).

Anal. Calcd. for C₂₅ H₃₇ N₃ O₃.2C₄ H₄ O₄ : C, 60.01; H, 6.88; H, 6.37.Found: C, 60.05; H, 6.97; N, 6.32.

1.3 g of the second crop bis-maleate salt from above was dissolved in 60ml warm H₂ O and basified with 5 ml 2 N NaOH. The resulting solid wasrecovered by filtration, washed with water and dissolved in CH₂ Cl₂. TheCH₂ Cl₂ solution was dried (K₂ CO₃) and evaporated to give the freeamine as a white solid. Crystallization from EtOAc furnished the purebase as a monohydrate, mp 82°-84°.

Anal. Calcd. for C₂₅ H₃₇ N₃ O₃.H₂ O: C, 67.39; H, 8.82; N, 9.43; H₂ O,4.21. Found: C, 67.22; H, 8.65; N, 9.13; H₂ O, 4.02.

EXAMPLE 33(S)-1-[2-(4-(2-Hydroxy-3-isopropylaminopropoxy)phenoxy)ethyl]-4-(2-methoxyphenyl)piperazineand its bis-maleate salt

9.33 g 4 N NaOH (37.3 mmol) was added to a solution of 8.4 g (37.3 mmol)(S)-1-(4-hydroxyphenoxy)-2-hydroxy-3-isopropylaminopropane and 9.0 g(35.4 mmol) 1-(2-chloroethyl)-4-(2-methoxyphenyl)piperazine in 75 mlDMSO and the mixture was heated at 60° for 90 min. After cooling, themixture was diluted with 200 ml 0.5 N NaOH and extracted using benzene(3×200 ml). The organic extracts were washed in turn with H₂ O (2×100ml) and then were combined, dried (K₂ CO₃) and evaporated.

8.6 g (74 mmol) of maleic acid was added to a solution of the residue in40 ml MeOH and EtOAc was added to the cloud point. The compound did notcrystallize and thus the solvents were removed in vacuo. The crude saltwas redissolved in EtOH (40 ml) and EtOAc was again added just to thecloud point. The resulting crude solid was recrystallized fromEtOH-EtOAc to give essentially pure product as its bis-maleate salt, mp105°-110°. The analytically pure salt [mp 115°-117°; [α]_(D) ²⁵ -10.4°(c, 1.0, MeOH]was obtained from the same solvents.

Anal. Calcd. for C₂₅ H₃₇ N₃ O₄.2C₄ H₄ O₄ : C, 58.66; H, 6.71; N, 6.22.Found: C, 58.52; H, 6.77; N, 6.50.

0.7 g of the bis-maleate salt was dissolved in 5 ml H₂ O and basifiedwith 3 ml 1 N NaOH. The resulting crude free base was filtered off anddried. Crystallization from ether afforded the analytically purematerial, mp 89°-90.5°.

Anal. Calcd. for C₂₅ H₃₇ N₃ O₄ : C, 67.69; H, 8.41; N, 9.47. Found: C,67.97; H, 8.56; N, 9.42.

EXAMPLE 34 1-(2-Bromoethoxy)-4-(2-propenyloxy)benzene

To a solution of 45.0 g of 4-(2-bromoethoxy)-phenol (0.207 mol) in 310ml of acetone was added 54 ml of allyl bromide (0.619 mol) and 45.0 g ofanhydrous potassium carbonate (0.326 mol). The reaction mixture wasstirred under reflux for eleven hours and was cooled and poured into2500 ml of water. The mixture was extracted twice with 3:1ether:methylene-chloride, and the organic layers were washed twice with1 N NaOH, once with H₂ O, dried (Na₂ SO₄) and concentrated to drynessunder reduced pressure to yield a colorless oil. The analytical samplewas prepared by crystallization from hexane to give colorless crystals,mp 27°-28°.

Anal. Calcd. for C₁₁ H₁₃ BrO₂ : C, 51.38; H, 5.10; Br, 31.08. Found: C,51.63; H, 5.27; Br, 31.22.

EXAMPLE 35 (R,S)-1-(2-Bromoethoxy)-4-(2,3-epoxypropoxy)benzene

To a solution of 48.0 g of 1-(2-bromoethoxy)-4-(2-propenyloxy)benzene(0.186 mol) in 960 ml of acetone and 125 ml of water was added 2.0 ml of70% perchloric acid. To this stirred reaction mixture was addedportionwise over a 12 min period 38.4 g (0.278 mol) of N-bromoacetamide.The stirred reaction mixture was maintained at 22° for 21/2 hr and wasthen treated with solid NaHSO₃ until a negative starch-KI reaction wasachieved. The acetone was next removed in vacuo, and the residue wasdiluted with 1 l of water. The mixture was extracted twice withmethylene chloride and the organic layers were washed with water, dried(Na₂ SO₄) and concentrated to dryness under reduced pressure to yieldthe bromohydrin intermediate as a red oil.

The crude oil was redissolved in 1300 ml methanol and treated with 375.0ml of 1 N NaOH. The reaction mixture was allowed to stand for two hoursat room temperature under argon at which time the methanol was removedin vacuo and the residue was diluted with 1 l of water. The resultingprecipitate was filtered and washed well with water. It was thenredissolved in methylene chloride, dried (Na₂ SO₄), and concentrated todryness under reduced pressure to yield a pale yellow solid. The crudesolid was chromatographed on a column of Florisil using 1:1 C₆ H₆ -CH₂Cl₂ as eluent. The eluted fractions were combined and concentrated todryness to yield a yellow solid, mp 50°-61.5°. The solid was thenrechromatographed through a column of Florisil in benzene and elutedagain with 1:1 C₆ H₆ -CH₂ Cl₂ to yield a colorless solid, mp 56°-62°.Crystallization from ether:hexane yielded the end product as colorlesscrystals, mp 59°-62°. A second crop from the mother liquors yieldedproduct, mp 58.5°-61.5°. Crystallization from ether:hexane gave theanalytical sample, mp 62°-64°.

Anal. Calcd. for C₁₁ H₁₃ BrO₃ : C, 48.37; H, 4.80; Br, 29.26. Found: C,48.40; H, 4.86; Br, 29.32.

EXAMPLE 36(R,S)-1-(2-Bromoethoxy)-4-[2-hydroxy-3-isopropylaminopropoxy]benzene

To a stirred suspension of 15.0 g of(R,S)-1-(2-bromoethoxy)-4-(2,3-epoxypropoxy)benzene (0.055 mol) in 150ml of methanol was added 10.2 ml of isopropylamine (0.122 mol). Thereaction mixture was stirred and heated in an argon atmosphere at 55°for 3 hours and was then cooled to room temperature. The solvent wasremoved in vacuo to yield a pale yellow oil which crystallized onstanding. The product was crystallized twice from methylenechloride-hexane to afford the end product as colorless crystals, mp83°-87.5°.

Crystallization from methylene chloride-hexane gave the analyticalsample, mp 87°-89.5°.

Anal. Calcd. for C₁₄ H₂₂ BrNO₃ : C, 50.61; H, 6.67; N, 24.05; Br, 4.22.Found: C, 50.50; H, 6.71; N, 24.05; Br, 4.09.

EXAMPLE 37(R,S)-1-(4-Benzyloxyphenoxy)-2-hydroxy-3-isopropylaminopropane

To a solution of 200 g (1 mol) p-benzyloxyphenol in 2.5 DMSO was added375 ml 4 N NaOH followed by 216 ml of epichlorohydrin and the resultingmixture was stirred at room temp for 3 hr. The reaction was poured into6 l of an ice-water mixture and extracted with CH₂ Cl₂ (3×1 l). Theorganic extracts were washed in turn with H₂ O (3×500 ml), and then werecombined, dried (Na₂ SO₄) and evaporated to dryness. The resulting crudesolid was crystallized from ether to give(R,S)-1-(4-benzyloxyphenoxy)-2,3-epoxypropane in two crops. A solutionof 135 g (0.527 mol) of the above epoxide in 1 l MeOH containing 135 mlisopropylamine was refluxed for 90 minutes. The solvents were thenremoved under reduced pressure and the residual solid was trituratedwith ether to give the end product amine, mp 99°-100°. A second crop, mp98°-100°, was obtained by concentration of the ether extracts.

A small sample (1 g) of the second crop was recrystallized from ether togive the analytical sample, mp 100°-101°.

Anal. Calcd. for C₁₉ H₂₅ NO₃ : C, 72.35; H, 7.99; N, 4.44. Found: C,72.42; H, 7.99; N, 4.41.

EXAMPLE 38 (R,S)-1-(4-Hydroxyphenoxy)-2-hydroxy-3-isopropylaminepropane

(R,S)-1-(4-Benzyloxyphenoxy)-2-hydroxy-3-isopropylaminopropane (124 g)in MeOH (1 l) containing 5 g 10% Pd/C was hydrogenated (21°; atmosphericpressure). The uptake of H₂ (total 9.6 l) essentially stopped after 1hr. The catalyst was removed by filtration (Celite) and the filtrate wasconcentrated to dryness in vacuo. The resulting solid residue wascrystallized from ethanol to give the end product, mp 158°-159°. Asecond crop of product was obtained by concentration of the motherliquor.

Anal. Calcd. for C₁₂ H₁₉ NO₃ : C, 63.98; H, 8.50; N, 6.22. Found: C,64.03; H, 8.62; N, 6.09.

EXAMPLE 39(R,S)-1-[2-(4-(2-Hydroxy-3-(isopropylaminopropoxy)phenoxy)ethyl]-4-phenylpiperazineand its bis-maleate salt Method A - From the product of Example 36

A stirred mixture of 9.0 g of(R,S)-1-(2-bromoethoxy)-4-[2-hydroxy-3-isopropylaminopropoxy]benzene(0.027 mol) and 9.0 g (0.055 mol) of N-phenylpiperazine in 135 ml ofethanol was refluxed under argon for seven hours. The reaction mixturewas then cooled to room temp and the solvent was removed in vacuo. Theresidue was dissolved in benzene and washed twice with 1 N NaOH solutionand once with water. The dried (Na₂ SO₄) organic layer was concentratedto dryness under reduced pressure to yield a crude solid which wascrystallized twice from ether:hexane to yield the end product base ascolorless crystals, mp 182.5°-186.5°. Crystallization from methylenechloride-ether gave the analytical sample, mp 87°-89.5°.

Anal. Calcd. for C₂₄ H₃₅ N₃ O₃ : C, 69.70; H, 8.53; N, 10.16. Found: C,69.57; H, 8.45; N, 10.02.

A solution of 7.70 g (18.6 mmol) of the free base in 150 ml of absoluteethanol was treated with a solution of 5.10 (43.9 mmol) of maleic acidin 80 ml of absolute ethanol. The reaction mixture was heated on thesteam bath for two minutes and was then allowed to cool slowly to roomtemp. The solvent was removed in vacuo to yield a pale yellow solidwhich was crystallized twice from methanol-ethyl acetate to yield thebis-maleate salt as pale yellow crystals, mp 141.5°-144°.Crystallization from methanol-ethyl acetate gave the analytical sampleas colorless crystals, mp 142°-144°.

Anal. Calcd. for C₃₂ H₄₃ N₃ O₁₁ : C, 59.52; H, 6.71; N, 6.51. Found: C,59.56; H, 6.94; N, 6.53.

EXAMPLE 40 The end product of Example 39 Method B - From the end productof Example 38

To a stirred mixture of(R,S)-1-(4-hydroxyphenoxy)-2-hydroxy-3-isopropylaminopropane (4.5 g; 20mmol) and 1-(2-chloroethyl)-4-phenylpiperazine (4.6 g; 20.5 mmol) in 35ml DMSO was added 5.0 ml 4 N NaOH (20 mmol). After stirring 3 hrs at60°, the reaction mixture was cooled and partitioned between CH₂ Cl₂ anddilute NaOH solution. The layers were separated and the aqueous layerwas re-extracted with CH₂ Cl₂ (2x). The organic layers were then washedin turn with H₂ O (2x) and were combined, dried (K₂ CO₃) and evaporatedin vacuo.

The crude free base thus obtained was dissolved in 75 ml MeOH containing4.6 g (40 mmol) maleic acid and EtOAc was added portionwise to theboiling solution to the cloud point. After the solution was chilled, theresulting solid was recovered by filtration to give the bis-maleatesalt. Recrystallization from MeOH-EtOAc furnished the pure salt, mp143.5°-144°.

EXAMPLE 41(S)-1-[6-(4-(2-Hydroxy-3-isopropylaminopropoxy)-phenoxy)hexyl]-4-piperazine

A solution of 25 g (0.128 mol) of 6-bromohexanoic acid and 12.7 g (0.125mol) of triethylamine in 150 ml of ether was cooled (0°) and treateddropwise with 28.5 g (0.263 mol) of ethyl chloroformate. The reactionmixture was stirred for 1 hr and then filtered. To the cooled (0°)filtrate was added a solution of 21 g (0.129 mol) of N-phenylpiperazinein 50 ml of ether. The reaction mixture was then allowed to warm to roomtemperature for 30 min and was then washed once with 1 N NaOH solutionand once with water. The dried (Na₂ SO₄) organic layers wereconcentrated in vacuo to give a yellow oil which was dissolved inbenzene and chromatographed on silica gel (500 g). The column was elutedwith benzene-ethyl acetate mixtures, the product appearing in thebenzene-ethyl acetate (8.5:1.5) mixtures. Evaporation of those combinedmixtures gave 1-(6-bromohexanoyl)-4-phenylpiperazine.

This material (4.55 g, 13.2 mmol) was added to a solution of 4.0 g (13.2mmol) of(S)-1-(4-hydroxyphenoxy)-2-hydroxy-3-N-mesylisopropylaminopropane in 30L ml of dimethylsulfoxide. To this stirred solution was added 3.33 ml of4 N NaOH solution and the reaction mixture was heated at 70° for 30 min.It was then cooled, diluted with water and extracted three times withethyl acetate. The organic layers were washed once in 1 N NaOH solution,twice with water, dried (Na₂ SO₄) and evaporated to give theintermediate condensation product as an oil.

To this material (7.4 g, 13.2 mmol) in 62 ml of dry benzene was addeddropwise 42 ml of 70% sodium bis(2-methoxyethoxy)-aluminum hydride inbenzene (Red-al). The reaction mixture was refluxed for 80 min and wasthen cooled and treated with 40 ml of 2 N NaOH solution followed by 100ml of H₂ O. The mixture was extracted three times with benzene and theorganic layers were washed twice with water, dried (Na₂ SO₄) andevaporated. The residue was crystallized from methylene chloride-etherto yield the end product (free base), mp 75°-76°. Crystallization fromacetone-hexane gave the analytical sample, mp 78°-79°; [α]_(D) ²⁵ -3.52°(c, 1.0, CHCl₃).

Anal. Calcd. for C₂₈ H₄₃ N₃ O₃ : C, 71.61; H, 9.23; N, 8.95. Found: C,71.69; H, 9.22; N, 8.79.

EXAMPLE 42(S)-1-[6-[4-(2-Hydroxy-3-isopropylaminopropoxy)phenoxy]hexyl]-4-phenylpiperazinedihydrochloride.

A solution of 4.265 g (9 mmol) of free base of Example 41 in 60 ml ofethanol was treated with 3.5 ml of 5.13 N ethanolic hydrogen chloride.The resulting solid was collected by filtration and washed with ethanolto give the end product, mp 183°-185°. Crystallization from ethanol gavethe analytical sample, mp 183°-184°; [α]_(D) ²⁵ -11.4° (c, 0.5, CH₃ OH).

Anal. Calcd. for C₂₈ H₄₃ N₃ O₃.2HCl: C, 61.98; H, 8.36; Cl, 13.07; N,7.74. Found: C, 61.69; H, 8.30; Cl, 12.85; N, 7.61.

EXAMPLE 43(S)-1-[11-(4-(2-Hydroxy-3-isopropylaminopropoxy)phenoxy)undecanyl]-4-phenylpiperazine

To a stirred solution of 13.26 g of 11-bromoundecanoic acid (50 mmol) in75 ml of ether was added 6.95 ml (50 mmol) of triethylamine. The stirredsolution was cooled to 0° and 4.80 ml of ethyl chloroformate (50 mmol)was added dropwise. The mixture was stirred for one hour at 0° and theresulting precipitate was filtered and washed with ether.

The filtrate was recooled to 0° and a solution of 8.10 g (50 mmol) ofN-phenylpiperazine in 25.0 ml of ether was added with stirring. Aprecipitate formed and the ice bath was removed. Stirring was continuedfor an additional 25 minutes at room temp. and the material was thencollected by filtration and was washed well with ether to yieldcolorless crystals, mp 70°-74°. Crystallization from methylenechloride:ether gave 1-(11-bromoundecanoyl)-4-phenylpiperazine ascolorless crystals, mp 73°-75.5°. Crystallization from methylenechloride-ether gave the analytical sample, mp 73.5°-75.5°.

Anal. Calcd. for C₂₁ H₃₃ BrN₂ O: C, 61.61; H, 8.13; N, 6.84; Br, 19.52.Found: C, 61.77; H, 8.14; N, 6.72; Br, 19.47.

To a stirred solution of 3.03 g (10 mmol) of(S)-1-(4-hydroxyphenoxy)-2-hydroxy-3-N-mesylisopropylaminopropane and4.09 g of 1-(11-bromoundecanoyl)-4-phenylpiperazine (10 mmol) in 20 mlof dimethylsulfoxide was added 3.0 ml (12 mmol) of 4 N NaOH solution.The reaction mixture was stirred and heated at 75°-78°, under argon for35 minutes and was then cooled to room temperature and diluted with 100ml of cold water. The mixture was extracted twice with ethyl acetate andthe organic layers were washed once with 1 N NaOH, twice with water, anddried (Na₂ SO₄). The solvent was evaporated to dryness under reducedpressure to yield a colorless oil.

To a stirred solution of 6.50 g of this oil (10.4 mmol) in 55.0 mlbenzene was added 36.6 ml of 70% sodium bis(2-methoxyethoxy)-aluminumhydride in benzene (Red-al). The reaction mixture stirred under reflux(argon atmosphere) for 21/4 hr, then cooled to room temp. and treatedwith 35 ml of 2 N NaOH and 100 ml of water. The mixture was extractedtwice with benzene and the organic layers were washed twice with water,dried (K₂ CO₃), and concentrated to dryness under reduced pressure toyield a colorless solid. The material was crystallized from acetone toyield the end product (free base) as colorless crystals, mp 99°-103°.Crystallization from acetone gave the analytical sample, mp 101.5°-104°;[α]_(D) ²⁵ -2.82° (c, 1.13, CHCl₃).

Anal. Calcd. for C₃₃ H₅₃ N₃ O₃ : C, 73.42; H, 9.90; N, 7.79. Found: C,73.62; H, 9.91; N, 7.62.

EXAMPLE 44(S)-1-[11-[4-(2-Hydroxy-3-isopropylaminopropoxy)phenoxy]undecanyl]-4-phenylpiperazinedihydrochloride.

To a warm (40°) solution of 2.83 g (5.2 mmol) of the free base ofExample 43 in 95 ml of absolute ethanol was added at a rapid dropwiserate with swirling 2.03 ml (10.4 mmol) of 5.13 N ethanolic hydrogenchloride solution. The mixture was then cooled to 0° and the resultingcolorless crystals were collected by filtration to yield the endproduct, mp 193°-196°. Crystallization from ethanol gave the analyticalsample, mp 193.5°-196°; [α]_(D) ²⁵ -7.52° (c, 0.5, CH₃ OH).

Anal. Calcd. for C₃₃ H₅₃ N₃ O₃.2HCl: C, 64.69; H, 9.05; N, 6.86; Cl,11.57. Found C, 64.60; H, 9.10; N, 6.88; Cl, 11.37.

EXAMPLE 45(S)-1-(2-Hydroxyphenoxy)-2-hydroxy-3-N-mesylisopropylaminopropane

39.9 g (0.362 mol) of catechol was added to a solution of 14.5 NaOH (362mmol) in 45 ml H₂ O and the mixture was stirred under argon. The pastymixture was diluted with 100 ml of DMSO and after 10 min, a solution of52.3 g (0.181 mol) of(S)-1-mesyloxy-2-hydroxy-3-N-mesylisopropylaminopropane in 100 ml ofDMSO was added. The solution was stirred at 80° under argon for 21/2 hrand then it was cooled and diluted with 400 ml 1 N NaOH. The solutionwas extracted with CH₂ Cl₂ (3×250 ml) and the organic extracts werebackwashed (1x) with dilute NaOH solution. The combined basic aqueousextracts were acidified using 70 ml concentrated HCl and extracted withCH₂ Cl₂ (2×500 ml). The organic extracts were then washed in turn withwater (5×500 ml) and then were combined, dried (Na₂ SO₄) and evaporatedto give the essentially pure monoalkylated end product as an oil.

EXAMPLE 46(S)-1-[2-(3-Bromopropoxy)phenoxy]-2-hydroxy-3-N-mesylisopropylaminopropane.

A mixture of 15 g (49.5 mmol)(S)-1-(2-hydroxy-phenoxy)-2-hydroxy-3-N-mesylisopropylaminopropane, 30.6g (˜150 mmol) 1,3-dibromopropane, and 10.6 g K₂ CO₃ in 60 ml acetone wasstirred under reflux overnight. The reaction was diluted with water andextracted with CH₂ Cl₂ (4x). The CH₂ Cl₂ extracts were washed in turnwith 1 N NaOH solution and then were combined, dried (Na₂ SO₄), andconcentrated to dryness in vacuo to give 18.5 g of an oil. The oil waschromatographed on 200 g silica gel made up in hexane. The column waseluted successively with hexane, benzene, benzene-Et₂ O (19:1),benzene-Et₂ O (9:1) and benzene-Et₂ O (3:1). The fractions eluted withbenzene-Et₂ O (9:1) and (3:1) were combined and evaporated to dryness togive chromatographically pure product, mp 95°-96°. Crystallization fromEtOAc-hexane furnished the pure compound, mp 98°-99° .

The analytical sample, mp 98°-100°; [α]_(D) ²⁵ -4.99° (c, 1.0, CHCl₃)was obtained from smaller experiment using the same solvent system.

Anal. Calcd. for C₁₆ H₂₆ BrNO₅ S: C, 45.29; H, 6.18; N, 3.30; S, 7.56;Br, 18.83. Found: C, 14.80; H, 6.16; N, 3.30; S, 7.60; Br, 18.32.

EXAMPLE 47(S)-1-[3-(2-(2-Hydroxy-3-N-mesylisopropylaminopropoxy)-phenoxy)propyl]-4-phenylpiperazine

A mixture of 10.5 g (24.7 mmol)(S)-1-[2-(3-bromopropoxy)phenoxy]-2-hydroxy-3-N-mesylisopropylaminopropaneand N-phenylpiperazine (4.42 g; 27.3 mmol) in 50 ml ethanol was left atroom temperature for ˜3 days. Tlc showed the reaction to be ˜50%completed but the ratio of starting materials and product did not changeafter 4 hrs at reflux. 4.0 ml 4 N NaOH was added and the reaction heatedfor 2 hours, and than an additional 1.5 ml 4 N NaOH [total used=22 equiv(95% of theory)] was added. After another 60 min at reflux, the reactionwas cooled and evaporated. The residue was diluted with water andextracted with EtOAc. The EtOAc extract was dried (K₂ CO₃) andevaporated to give an oil. The oil was chromatographed over 200 g silicagel. The column was eluted with benzene and benzene-EtOAc mixtures(19:1; 9:1; 3:1 and 1:1) and then the product was eluted from the columnusing ethyl acetate to give the end product. Crystallization from etherafforded product in two crops, (mp 84°-85° and 82°-84°).

Recrystallization of a small amount from ether (2x) furnished theanalytically pure material, mp 85°-86°; [α]_(D) ²⁵ -1.0° (c, 1.0,CHCl₃).

Anal. Calcd. for C₂₆ H₃₉ N₃ O₅ S: C, 61.76; H, 7.77; N, 8.31; S, 6.34.Found: C, 61.92; H, 7.85; N, 8.26; S, 62.9.

EXAMPLE 48(S)-1-[3-(2-(2-Hydroxy-3-N-mesylisopropylaminopropoxy)-phenoxy)propyl]-4-phenylpiperazineand its dihydrochloride

22 ml Red-al (60% solution in benzene) was added dropwise at first andthen, once the bubbling had ceased, more rapidly to a stirred solutionof 6.2 g (12.25 mmol)(S)-1-[3-(2-(2-hydroxy-3-N-mesylisopropylaminopropoxy)phenoxy)propyl]-4-phenylpiperazinein 100 ml dry benzene. The reaction was refluxed for 5 hrs under argonand then it was left overnight at room temp. Excess reagent wasdecomposed by the dropwise addition of 25 ml 2 N NaOH and then it wasdiluted with water (25 ml). The benzene layer was separated and theaqueous layer was extracted with benzene (2x). The benzene layers werewashed in turn with 2 N NaOH solution (1x) and brine (2x). The combinedextracts were dried (K₂ CO₃) and evaporated under reduced pressure togive the crude amine. Crystallization from acetone-hexane gave solids intwo crops, which when recrystallized from the same solvent systemafforded the pure amine, mp 87°-88°.

Anal. Calcd. for C₂₅ H₃₇ N₃ O₃ : C, 70.23; H, 8.72; N, 9.83. Found: C,70.27; H, 8.74; N, 9.70.

3.5 g of the amine in 150 ml absolute EtOH was treated with 2.2 molarequivalents of ethereal HCl and then the solution was diluted to 400 mlwith ether. The resulting crystalline solid was collected by filtrationto give the dihydrochloride salt, mp 192°-193°.

The analytically pure material, mp 192°-193°; [α]_(D) ²⁵ -5.39° (c, 1.0,H₂ O) was obtained by recrystallization from EtOH.

Anal. Calcd. for C₂₅ H₃₇ N₃ O₃.2HCl: C, 59.99; H, 7.85; N, 8.40; Cl,14.17. Found: C, 60.20; H, 8.13; N, 8.25; Cl, 14.28.

EXAMPLE 49(S)-1-[6-(2-(2-Hydroxy-3-N-mesylisopropylamino)propoxy)phenoxy)hexanoyl]-4-phenylpiperazine

8.0 ml 4 N NaOH (32 mmol) was added to a solution of 9.5 g (31.35 mmol)(S)-1-(2-hydroxyphenoxy)-2-hydroxy-3-N-mesylisopropylaminopropane and10.9 (32 mmol) 1-(6-bromohexanoyl)-4-phenylpiperazine in 70 ml DMSO andthe mixture was heated at 80° under argon for 50 min. The reaction wascooled and poured into 500 ml H₂ O and extracted with benzene (2×250ml). The benzene extracts were washed in turn with 1 N NaOH (2x) and H₂O (1x), and then were combined, dried (K₂ CO₃) and evaporated to give anoil. The oil was chromatographed on 200 g silica gel made up in benzene.The fractions eluted with EtOAc:benzene (1:1 and 3:2) were combined andevaporated to yield essentially pure product as an oil.

A small sample was purified for analysis by preparative tlc.

Anal. Calcd. for C₂₉ H₄₃ N₃ O₆ S: C, 62.01; H, 7.71; N, 7.48; S, 5.71.Found: C, 62.02; H, 7.98; N, 7.23; S, 5.46.

EXAMPLE 50(S)-1-[6-(2-(2-Hydroxy-3-isopropylaminopropoxy)phenoxy)hexyl]-4-phenylpiperazineand its fumarate salt.

In an inert atmosphere 70 ml Red-al (70% solution in benzene) was addedcautiously at first and then more rapidly to a stirred solution of 11.4g (20.28 mmol)(S)-1-[6-(2-(2-hyroxy-3-N-mesylisopropylaminopropoxy)phenoxy)hexanoyl]-4-phenylpiperazinein 100 ml dry benzene. The reaction was then heated and stirred underreflux for 90 minutes. 50 ml 2 N NaOH solution was then added carefullyto the cooled reaction mixture, followed by 100 ml H₂ O and 300 mlbenzene. The aqueous layer was separated and extracted with benzene(2×300 ml). The benzene layers were then backwashed in turn with water(2×100 ml). The pooled organic extracts were dried (K₂ CO₃) andconcentrated to give an oil which solidified when it was evaporated invacuo from acetone. The residue was crystallized from acetone-hexane togive the pure amine, mp 69°-71°. Recrystallization from acetone-hexanegave the analytical sample, mp 69°-71°; [α]_(D) ²⁵ +3.5° (c, 1.0,CHCl₃).

Anal. Calcd. for C₂₈ H₄₃ N₃ O₃ : C, 71.61; H, 9.23; N, 8.95. Found: C,71.82; H, 9.38; N, 8.80.

4.0 g (8.55 mmol) of the amine and 0.99 g (8.55 mmol) of fumaric acidwere dissolved in 25 ml MeOH at room temp. and then the solution wasdiluted with 50 ml EtOAc. The resulting fumarate salt which slowlycrystallized from solution was collected by filtration and was washedwith EtOAc to yield the salt, mp 163°-165°.

The analytically pure specimen of fumarate, mp 164°-165° was obtained byrecrystallization from MeOH-EtOAc.

Anal. Calcd. for C₂₈ H₄₃ N₃ O₃.C₄ H₄ O₄ : C, 65.62; H, 8.09; N, 7.17.Found: C, 65.36; H, 7.92; N, 7.12.

EXAMPLE 51 Bromohydrins from 4-allyloxyphenylacetic acid

A solution of 5.00 g (0.0260 mol) of 4-allyloxyphenylacetic acid in 25ml of acetone and 8 ml of water was cooled in an ice bath and treatedwith 5.00 g (0.0362 mol) of N-bromosuccinimide (NBS). The resultingmixture was allowed to warm to room temperature slowly and after 2.5hour, an additional 0.5 g. of NBS was added. After a total of 3.5 hour,the reaction mixture was diluted with 150 ml of water and a small amountof citric acid. The product was extracted with methylene chloride andafter evaporation of the solvent, gave a crude solid. Recrystallizationfrom ethyl acetate-hexane gave a mixture of bromohydrins containing theend-products, m.p. 85°-99°. Several crystallizations from ethylacetate-hexane gave an analytically pure sample of one of the isomers,m.p. 128°-133°.

EXAMPLE 52 (R,S)-4-(2,3-Epoxypropoxy)phenylacetic acid Method A

A solution of 1.66 g (5.74 mmol) of the mixture of bromohydrins obtainedin Example 51 in 100 ml of methanol and 5 ml of 4 N sodium hydroxidesolution was stirred at room temperature for 2 hours. The resultingmixture was diluted with 1 M citric acid to pH 3 and extracted withmethylene chloride. Crystallization of the crude product frommethanol-water give the endproduct. Recrystallization frommethanol-ether gave the analytical sample, m.p. 73°-77°.

Anal. (C₁₁ H₁₂ O₄), C, H.

Method B

A solution of 2.00 g (10.4 mmol) of 4-allyloxyphenylacetic acid and 1.6g. of NBS in 10 ml. of acetone and 4 ml. of water was stirred 30 minutesat 0° and allowed to warm to room temperature. After an hour 0.2 g ofNBS were added and after a total of 2.5 hour, the mixture was cooled inan ice bath and 5.5 ml of 4 N sodium hydroxide were added. The mixturewas warmed to room temperature, stirred one hour, and diluted withwater, ice, and 10% citric acid solution. The solution was extractedwith methylene chloride and the methylene chloride layers afforded theproduct as an off-white solid, m.p. 72°-76°.

EXAMPLE 53 Methyl 4-allyloxyphenyl acetate

A mechanically stirred suspension of 101.54 g (0.528 mol) of4-allyloxyphenylacetic acid, 70 ml (1.12 mol) of iodomethane, and 100 g(0.756 mol) of potassium carbonate in 210 ml. of hexamethylphosphoramidewas left at room temperature overnight. The resulting mixture wasdiluted with 1 liter of ether and the ethereal solution was washed 3×300ml water and 1×300 ml. saturated brine. The ethereal solution wasconcentrated and the product was distilled to give a colorless oil, b.p.120°-135°/0.05 mm.

EXAMPLE 54 (R,S)-Methyl-4-(2,3-epoxypropoxy)phenyl acetate Method A

To an ice cold solution of 4.50 g (0.0216 mol) of the product of Example52 in 20 ml of dry methanol was added an excess of etherealdiazomethane. Evaporation of the resulting solution to dryness gave theester.

Method B

A solution of 25.25 g (0.122 mol) of the product of Example 53 and 62 g(0.359 mol) of m-chloroperbenzoic acid in 200 ml of methylene chloridewas stirred at room temperature. After 24 hours the reaction mixture waspoured into 200 ml. of water made basic with 45% NaOH. The aqueous layerwas extracted 2×300 ml CH₂ Cl₂ and the combined organic layers werewashed 2×200 ml. H₂ O and 1×200 ml. brine. The crude product wasevaporatively distilled to give the end-product, b.p. 180°-185°/0.1 mm.A portion was purified by preparative layer chromatography anddistilled.

Anal. (C₁₂ H₁₄ O₄) C, H.

EXAMPLE 55 Methyl 4-(2-hydroxy-3-isopropylaminopropoxy)phenyl acetate

A solution of 55.29 g (0.249 mol) of the product of Example 54 and 27.0ml (0.334 mol) of isopropylamine in 200 ml. of methanol was stirredovernight at room temperature. The mixture was concentrated and theresidue was dissolved in excess 1 M citric acid. The aqueous solutionwas washed 3×100 ml. CH₂ Cl₂ and was made basic by addition of 45% NaOHfollowed by extraction with 4×100 ml CH₂ Cl₂. The combined extracts werewashed with saturated brine and were dried (K₂ CO₃). Evaporation gavethe end-product, m.p. 72°-74°. Recrystallization from ether-hexane gavethe analytical sample, m.p. 72°-74°.

Anal. (C₁₅ H₂₃ NO₄) C, H, N.

EXAMPLE 56 6-(4-Phenyl-1-piperazinyl)hexamide

A suspension of 9.85 g (0.060 mol) of phenylpiperazine, 11.6 g (0.060mol) of 6-bromohexamide, and 6.35 g (0.060 mol) of sodium carbonate in90 ml of toluene was heated reflux overnight. On cooling, the mixturewas filtered and the filtrate was washed with water. Evaporation andcrystallization of the crude product from methylene chloride-hexane gaveanalytically pure product, m.p. 129°-130°.

Anal. (C₁₆ H₂₅ N₃ O) C, H, N.

EXAMPLE 57 11-(4-Phenyl-1-piperazinyl)undecamide

A suspension of 13.1 g (0.0795 mol) of phenylpiperazine, 20.86 g (0.0795mol) of 11-bromoundecamide and 8.45 g (0.0795 mol) of sodium carbonatein 120 ml of toluene was heated to reflux overnight. On cooling thesolid which separated was collected and triturated with water to givethe end-product, m.p. 128°-129°. Two recrystallizations fromethanol-ethyl acetate-hexane gave the analytical sample, m.p. 129°-130°.

Anal. (C₁₂ H₃₇ N₃ O) C, H, N.

EXAMPLE 58 1-(6-Aminohexyl)-4-phenylpiperazine

A solution of 38.3 g (0.139 mol) of the product of Example 56 in 400 mlof THF was treated with 500 ml. of 1 M borane in THF. The solution wasrefluxed for 8 hours and was cooled in an ice bath as 42 ml. of 12 Nhydrochloric acid was added carefully. The excess THF was evaporated,the aqueous solution was made basic with potassium carbonate and wasextracted with CH₂ Cl₂. Evaporation of the organic layers gave an oilwhich gave a single spot on silica gel TLC and was used in the neatexample without further purification. A portion was furthercharacterized as its dihydrochloride salt which crystallized fromethanol, m.p. 251°-253°.

Anal. (C₁₆ H₂₇ N₃.2HCl) C, H, N.

EXAMPLE 59 1-(11-Aminoundecyl)-4-phenylpiperazine

1-(11-aminoundecyl)-4-phenylpiperazine was prepared similarly startingwith 11.2 g (0.0322 mol) of the product of Example 57 to give theend-product as an oil. The dihydrochloride salt crystallized fromethanol, m.p. 197°-202°.

Anal. (C₂₁ H₃₇ N₃.2HCl) C, H; N, calcd. 10.39; fd 9.87.

EXAMPLE 604-[(2-Hydroxy-3-isopropylaminopropoxy)phenyl]-N-[2-(4-phenyl-1-piperazinyl)ethyl]acetamidedihydrochloride

An intimate mixture of 5.00 g (0.0178 mol) of the product of Example 55and 4.00 g (0.0190 mol) of 1-(2-aminoethyl)-4-phenylpiperazine* washeated to a bath temperature of 140°-145° overnight. The resulting solidwas triturated with etherbenzene to give a tan solid, m.p. 100°-106°.Conversion to the hydrochloride salt gave the dihydrochloride salt, m.p.173°-177°. Two recrystallizations from ethanol-ethyl acetate gave theanalytical sample, m.p. 178°-181°.

Anal. (C₂₆ H₃₈ N₄ O₃.2HCl) C, H, N, Cl.

EXAMPLE 614-[(2-Hydroxy-3-isopropylaminopropoxy)phenyl]-N-[6-(4-phenyl-1-piperazinyl)hexyl]acetamidedihydrochloride

An intimate mixture of 10.0 g (0.0384 mol) of the product of Example 58and 10.0 g (0.0355 mol) of the product of Example 55 was heated to abath temperature of 140° overnight. The crude brown solid was dissolvedin CH₂ Cl₂ and ether was added to precipitate a waxy solid. Thismaterial was passed through 50 g of grade III basic alumina eluting with1% methanolethyl acetate to give a colorless wax which displayed asingle spot on TLC. For conversion to the hydrochloride salt, a total of9.1 g of material obtained as above was divided into portions of 6 g and3.1 g and the 6 g portion was treated with excess hydrochloric acid andevaporated to dryness under high vacuum. This oil was taken up inalcohol and the 3.1 g portion was added. The resulting solution wasconcentrated and the residue was crystallized from ethanol-ether to givethe end-product, m.p. 124°-6° (decomp). The analytical sample wasobtained from ethanol-ether, m.p. 123°-126° (decomp).

Anal. (C₃₀ H₄₆ N₄ O₃.2HCl) C, H, N, Cl.

EXAMPLE 624-[[2-Hydroxy-3-(isopropylaminopropoxy)phenyl]-N-[11-(4-phenyl-1-piperazinyl)undecyl]acetamideand its dihydrochloride

An intimate mixture of 9.4 g (0.030 mol) of the product of Example 59and 8.45 g (0.030 mol) of the product of Example 55 were heatedovernight at a bath temperature of 140°. The resulting brown solid wascrystallized from methylene chloride-ether to give the free base, m.p.107°-109°. A second crop, m.p. 101°-105° was obtained from the filtrate.The two crops were combined and acidified with hydrochloric acid to givea solid which was recrystallized from isopropanol to give the salt, m.p.146°-150°.

Anal. (C₃₅ H₅₆ N₄ O₃.2HCl) C, H, N, Cl.

EXAMPLE 63 (S)-4-(Dihydroxypropoxy)phenylacetic acid, methyl ester

Sodium (2.3 g; 0.1 mol) was dissolved in 500 ml anhydrous MeOH and tothis was added a solution of 194.3 g (1.17 mol) 4-hydroxyphenylaceticacid, methyl ester in 250 ml MeOH followed by a solution of 167.3 g.(1.018 mol) (S)-1,2-epoxy-3-benzyloxypropane in 250 ml MeOH. Thesolution was stirred under reflux for 12 hours. The cooled solution wastreated with 8.3 ml (0.1 mol) of concentrated HCl and the methanol wasremoved in vacuo. The crude product was dissolved in benzene and washedwith 2×200 ml portions of cold 1 N NaOH solution and with H₂ O (1×300ml). The aqueous layers were backwashed with benzene (2×500 ml). Thecombined organic layers were dried (Na₂ SO₄) and evaporated in vacuo togive crude (S)-4-(3-benzyloxy-2-hydroxypropoxy)phenyl acetic acid,methyl ester.

The crude ester was then dissolved in 1 liter acetic acid containing 20ml concentrated HCl and the mixture was hydrogenated over 10 g 10% Pd/Cat normal temperature and pressure. After ˜100 minutes the absorption ofH₂ ceased abruptly (total uptake ˜23.3 liters). The catalyst was removedby filtration through Celite and the filtrate was evaporated to drynessunder reduced pressure. Since the product had undergone partialacetylation during the hydrogenolysis, it was dissolved in 1 liter 1 Nmethanolic HCl and left at room temperature for 1 hour. Evaporation ofthe solvent furnished the diol product as a white solid.

Crystallization of a small sample from ether-hexane furnished theanalytically pure material, m.p. 64°-66°; [α]_(D) ²⁵ +6.55° (c, 1.0,EtOH).

Anal. Calcd. for C₁₂ H₁₆ O₅ : C, 59.99; H, 6.71. Found: C, 60.14; H,6.75.

EXAMPLE 64 (R)-4-(2-Acetoxy-3-chloropropoxy)phenylacetic acid methylester

A stirred mixture of 223.3 g (0.916 mol)(S)-4-(2-dihydroxypropoxy)phenylacetic acid, methyl ester, 3.0 g benzoicacid (0.024 mol) and 165 g (1.375 mol) trimethylorthoacetate was heatedin an oil bath at 80°. Methanol was distilled from the reaction as itwas formed. After 30 minutes, the reaction was cooled and the productwas partitioned between 1 liter CH₂ Cl₂ and 300 ml cold saturated NaHCO₃solution. The organic extract was washed with an additional 200 mlNaHCO₃ solution and then aqueous layers were backwashed with CH₂ Cl₂(1×200 ml). The combined CH₂ Cl₂ extracts were dried and evaporated togive the cyclic orthoacetate.

Without further purification this material (0.895 mol) was dissolved in750 ml dry CH₂ Cl₂ followed by the addition of 151 ml (1.16 mol) oftrimethylchlorosilane. The resulting solution was heated at reflux ˜45minutes, whereupon the solvent and excess reagent was removed in vacuoto give the chloroacetate as an oil.

A small portion of the product (1 g) was purified for analysis bychromatography on silica gel to give 950 mg of pure material, [α]_(D) ²⁵-23.86° (c, 1.0, MeOH).

Anal. Calcd. for C₁₄ H₁₇ ClO₅ : C, 55.91; H, 5.70; Cl, 11.79. Found: C,55.90; H, 6.04; Cl, 11.50.

EXAMPLE 65 (R)-4-(2,3-Epoxypropoxy)phenylacetic acid

A cold solution of 63 g (1.575 mol) NaOH in 200 ml H₂ O was added withstirring over ˜15 minutes to a previously chilled solution (0°) of 135 g(0.45 mol) crude (R)-4-(2-acetoxy-3-chloropropoxy)phenylacetic acid,methyl ester in 500 ml MeOH. The reaction temperature did not exceed 10°during the addition, and was then maintained at 0°-5° for 50 minutes and10°-15° for an additional 20 minutes. The reaction was then rechilledand acidified using a solution of 32 ml concentrated H₂ SO₄ (1.15 equiv)in 200 ml. H₂ O. Some MeOH (˜250 ml) was removed by concentration of thereaction mixture in vacuo (bath temperature ˜25°) and then the solutionwas extracted with benzene (4×1 liter). The benzene layers were washedin turn with brine (1×250 ml) and were combined, dried (Na₂ SO₄) andconcentrated to 500 ml in vacuo. The benzene solution was warmed to ˜40°and diluted to the cloud point with warm hexane and then it was storedat 0° for several hours. The resulting crystalline material wascollected by filtration to give the epoxy acid end product, m.p.70°-72°; [α]_(D) ²⁵ +10.6° (c, 1.0, MeOH). Concentration of the motherliquors gave additional epoxy acid.

Anal. Calcd. for C₁₁ H₁₂ O₄ : C, 63.45; H, 5.81. Found: 63.27; H, 5.89.

EXAMPLE 66 (R)-4-(2,3-Epoxypropoxy)phenylacetic acid, cyanomethyl ester

20.8 g (0.1 mol) of (R)-4-(2,3-epoxypropoxy)phenylacetic acid wassuspended in 30 ml chloroacetonitrile and cooled to ˜10° in an ice waterbath. Triethylamine (16 ml) was added to the stirred mixture at a rapiddropwise rate. The cooling bath was removed and after 30 minutes thetemperature had risen to 30°. The temperature was maintained at 25°-30°by the intermittent use of the cooling bath. After 90 minutes thereaction was partitioned between toluene and H₂ O. The organic phase waswashed with saturated NaHCO₃ solution and with water and then theaqueous layers were backwashed with toluene. The toluene extracts werecombined, dried (MgSO₄) and evaporated to give the cyanomethyl ester endproduct as an oil. This material was used without further purification.

EXAMPLE 67(R)-4-(2,3-Epoxypropoxy)phenyl-N-[2-(4-phenyl-1-piperazinyl)ethyl]acetamide

A mixture of 10.7 g (52.1 mmol) 1-(2-aminoethyl)-4-phenylpiperazine and13.5 g (54.6 mmol) (R)-4-(2,3-epoxypropoxy)phenylacetic acid,cyanomethyl ester in 125 dry THF was stirred at room temperature for 40hours. The solvent was removed in vacuo and the residue was trituratedwith ether to give the crude amide as a white solid. The solid wasdissolved in 35 ml. CH₂ Cl₂ and placed on a column of 170 g Woelm basicalumina (grade 3) made up in CHCl₂. Elution with 1 liter CH₂ Cl₂furnished essentially pure material. Crystallization from acetoneafforded the end product, m.p. 124°-125°. A second crop, m.p. 121°-123°was obtained from the mother liquors.

The analytical sample had been obtained from a previous batch byrecrystallization from MeOH-H₂ O to give pure material, m.p. 123°-124°;[α]_(D) ²⁵ +5.64° (c, 1.0, MeOH).

Anal. Calcd. for C₂₃ H₂₉ N₃ O₃ ; C, 69.85; H, 7.39; N, 10.62. Found: C,69.62; H, 7.40; N, 10.51.

EXAMPLE 68(R)-4-(2,3-Epoxypropoxy)phenyl-N-[6-(4-phenyl-1-piperazinyl)hexyl]acetamide

A mixture of 9.4 g (38 mmol) (R)-4-(2,3-epoxypropoxy)phenylacetic acid,cyanomethyl ester and 9.4 g (36 mmol)1-(6-aminohexyl)-4-phenylpiperazine in 75 ml THF was stirred for 60hours at room temperature. The solvent was removed in vacuo and theresidue was triturated with warm ether to give the crude amide. 12.4 g.of the crude amide in 25 ml CH₂ Cl₂ was placed on a column of 125 gWoelm basic alumina (grade 3). Elution with 750 ml. CH₂ Cl₂ furnishedhomogenous (tlc) material which was crystallized from acetone to givepure amide, m.p. 108°-110°.

The analytical sample, m.p. 108°-110°, was prepared in a previous run bycrystallization from MeOH-H₂ O.

Anal. Calcd. for C₂₇ H₃₇ N₃ O₃ : C, 71.81; H, 8.26; N, 9.30. Found: C,72.03; H, 8.13; N, 9.14.

EXAMPLE 69(S)-4-(2-Hydroxy-3-isopropylaminopropoxy)phenyl-N-[2-(4-phenyl-1-piperazinyl)ethyl]acetamideand its dihydrochloride salt

A solution of 10.86 g (27.5 mmol)(R)-4-(2,3-epoxypropoxy)phenyl-N-[2-(4-phenyl-1-piperazinyl)ethyl]acetamidein 200 ml methanol containing 100 ml isopropylamine was refluxed for 90minutes. The solution was evaporated to dryness and triturated with hotether to give the amine, m.p. 121°-123°.

Crystallization of a small portion from EtOH-EtOAc furnished theanalytically pure material, m.p. 123°-124°; [α]_(D) ²⁵ -0.8° (c, 1.0,MeOH).

Anal. Calcd. for C₂₆ H₃₈ N₄ O₃ : C, 68.69; H, 8.43; N, 12.32. Found: C,68.90; H, 8.66; N, 12.15.

A portion of the above amine (8 g) was dissolved in 50 ml EtOH andtreated with 35 ml 1.73 N methanolic HCl (60 mmol) and evaporated todryness. Residue was swirled down 2x from ethanol solution which removedthe excess HCl. The residue was then redissolved in 50 ml EtOH and theremaining portion of the above amine (4 g) was added. Upon the additionof 50 ml. EtOAc, crystals began to form to yield after cooling puredihydrochloride salt, m.p. 160°-162°; [α]_(D) ²⁵ -12.75°. A second crop159°-161° was obtained from the mother liquors.

Anal. Calcd. for C₂₆ H₃₈ N₄ O₃ : C, 59.20; H, 7.64; N, 10.62, Cl, 13.44.Found: C, 58.95; H, 7.49; N, 10.41; Cl, 13.62.

EXAMPLE 70(S)-4-(2-Hydroxy-3-isopropylaminopropoxy)phenyl-N-[6-(4-phenyl-1-piperazinyl)hexyl]acetamideand its maleate salt

A solution of 7.8 g(R)-4-(2,3-epoxypropoxy)phenyl-4-[6-(4-phenyl-1-piperazinyl)hexyl]acetamidein 175 ml methanol containing 75 ml isopropylamine was heated underreflux for 75 minutes. The solution was evaporated to dryness and theresidue was triturated with ether to give the amino-alcohol end product.Crystallization of the product from MeOH-EtOAc furnished pure material,m.p. 103°-104°.

The analytical specimen, m.p. 103°-104°; [α]_(D) ²⁵ -0.68° (c, 1.0,MeOH) was obtained from the same solvent mixture.

Anal. Calcd. for C₃₀ H₄₆ N₄ O₃ : C, 70.55; H, 9.08; N, 10.97. Found: C,70.55; H, 9.06; N, 10.93.

4.4 g (8.615 mmol) of the amine was dissolved in acetone (100 ml)containing 1.0 g (8.615 mmol) of maleic acid. The solution wasconcentrated to ˜75 ml and then was cooled to 0°. The resultingcrystalline salt was recovered by filtration to give the maleate, m.p.107°-108°. Recrystallization from acetone gave the pure salt, m.p.107°-108°; [α]_(D) ²⁵ -10.19° (c, 1% MeOH).

Anal. Calcd. for C₃₀ H₄₆ N₄ O₃.C₄ H₄ O₄ : C, 65.15; H, 8.04; N, 8.94.Found: C, 65.09; H, 8.15; N, 8.67.

EXAMPLE 71 N-(4-Hydroxyphenyl)-4-phenyl-1-piperazinepropanamide

A solution of 42.0 g (0.259 mol) of 1-phenylpiperazine and 40.0 g (0.245mol) of N-(4-hydroxyphenyl)-2-propenamide in 600 ml of ethanol washeated under reflux overnight and then cooled. The resulting precipitatewas collected and recrystallized from methanol to give the end productas colorless crystals, mp 172°-174°. The analytical sample was obtainedby recrystallization from ethanol and had mp 174°-176°.

Anal. Calcd. for C₁₉ H₂₃ N₃ O₂ : C, 70.13; H, 7.12; N, 12.91. Found: C,70.22; H, 7.09; N, 12.96.

EXAMPLE 72(R,S)-N-[4-(3-Chloro-2-hydroxypropoxy)phenyl]-4-phenyl-1-piperazinepropanamideHydrochloride

To a solution of 33.0 g (0.102 mol) ofN-(4-hydroxyphenyl)-4-phenyl-1-piperazinepropanamide in 120 ml of 1 Nsodium hydroxide was added 50 ml of epichlorohydrin. The reaction wasstirred at room temperature overnight and then extracted three timeswith chloroform. The solvent was removed under vacuum and thecrystalline residue was dissolved in 100 ml of concentrated hydrochloricacid. After standing at room temperature for 15 min the solution wasconcentrated to dryness under vacuum. The residue was recrystallizedfrom methanol using a Soxhlet thimble to give the end product ascolorless crystals, mp 242°-244°. Further recrystallization gave theanalytical sample, mp 243°-245°.

Anal. Calcd. for C₂₂ H₂₈ ClN₃ O₃.HCl: C, 58.15; H, 6.43; Cl, 15.61; N,9.25. Found: C, 58.16; H, 6.51; Cl, 15.90; N, 9.18.

EXAMPLE 73(R,S)-N-[4-[3-[(1-Methylethyl)amino]-2-hydroxypropoxy]phenyl]-4-phenyl-1-piperazinepropanamideBis-Maleate

A solution of 2.00 g (0.0044 mol) of(R,S)-N-[4-(3-chloro-2-hydroxypropoxy)phenyl]-4-phenyl-1-piperazinepropanamidehydrochloride and 20 ml of 1-methylethylamine in 20 ml of methanol washeated under reflux for 3 days and then concentrated under vacuum. Theresidue was mixed with dilute hydrochloric acid and the resultingsolution was washed several times with chloroform and then made basicwith sodium bicarbonate. The mixture was extracted several times withchloroform and the extracts were dried and concentrated to give(R,S)-N-[4-[3-[1-methylethyl)amino]-2-hydroxypropoxy]-phenyl]-4-phenyl-1-piperazinepropanamideas an amorphous solid. To a methanol solution of 0.80 g (0.0018 mol) ofthis solid was added 0.42 g (0.0036 mol) of maleic acid. The methanolwas removed under vacuum and the residue was recrystallized severaltimes from acetonitrile to give the end product as colorless crystals,mp 159.5°-161.5°.

Anal. Calcd. for C₂₅ H₃₆ N₄ O₃.2C₄ H₄ O₄ : C, 58.92; H, 6.59; N, 8.33.Found: C, 58.62; H, 6.78; N, 8.36.

EXAMPLE 74 6-Bromo-N-(4-hydroxyphenyl)hexanamide.

A mixture of 39.0 g (0.20 mol) of 6-bromohexanoic acid and 39.0 ml ofthionyl chloride was heated with a 90° oil bath until the temperature ofthe mixture had reached 80° and the initial gas evolution hadessentially stopped. Excess thionyl chloride was distilled from thereaction mixture under aspirator vacuum. Toluene (25 ml) was added andthis also was distilled under aspirator vacuum. The cooled reactionmixture was then added to a slurry of 43.6 g (0.40 mol) of 4-aminophenolin 500 ml of dioxane. After stirring for 30 min the mixture was filteredand the filtrate was concentrated under vacuum. The residue wasrecrystallized from trichloromethane using a soxhlet thimble to give theend product as tan crystals, mp 127°-129°. The analytical sample wasobtained from dichloromethane with charcoal; colorless crystals, mp126°-128°.

Anal. Calcd. for C₁₂ H₁₆ BrNO₂ : C, 50,36; H, 5.64; Br, 27.93; N, 4.89.Found: C, 50.47; H, 5.69; Br. 27.92; N, 4.68.

EXAMPLE 75 N-(4-Hydroxyphenyl)-4-phenyl-1-piperazinehexanamide

A solution of 45.7 g (0.16 mol) of6-bromo-N-(4-hydroxyphenyl)hexanamide, 26.0 g (0.16 mol) of1-phenylpiperazine and 22.4 ml (16.3 g, 0.16 mol) of triethylamine in400 ml of ethanol was heated under reflux overnight and thenconcentrated under vacuum. The residue was mixed with sodium bicarbonatesolution and trichloromethane. The resulting suspension was filtered.The solid was recrystallized from ethanol-water and then fromacetonitrile to give the end product as tan crystals, mp 157°-159°.

Anal. Calcd. for C₂₂ H₂₉ N₃ O₂ : C, 71.90; H, 7.95; N, 11.43. Found: C,71.65; H, 7.99; N, 11.38.

EXAMPLE 76(R,S)-N-[4-[3-[(1-Methylethyl)amino]-2-hydroxypropoxy]phenyl]-4-phenyl-1-piperazinehexanamide

A mixture of 38.1 g (0.104 mol) ofN-(4-hydroxyphenyl)-4-phenyl-1-piperazinehexanamide and 500 ml of 3 Nsodium hydroxide was stirred at room temperature for 45 min. To theresulting solution was added 76 ml of epichlorohydrin and stirring wascontinued overnight. The heterogeneous reaction mixture was extractedwith dichloromethane and the extracts were dried and concentrated togive 45.0 g of an oil. This was absorbed onto a column of 500 g ofsilica gel. Elution with 7 and 10% ethyl acetate in dichloromethane gavea colorless solid. A solution of 8.8 g of this colorless solid and 50 mlof 1-methylethylamine in 50 ml of methanol was heated under reflux for 4hr and concentrated under vacuum. The residue was mixed withdichloromethane and sodium bicarbonate solution and the organic layerwas dried and concentrated. The residue was precipitated several timesfrom dichloromethane-ether to give the end product as a colorlessamorphous solid, mp 125°-130°.

Anal. Calcd. for C₂₈ H₄₂ N₄ O₃ : C, 69.68; H, 8.77; N, 11.61. Found: C,69.64; H, 8.48; N, 11.49.

EXAMPLE 77(R,S)-N-[4-[3-[(1-Methylethyl)amino]-2-hydroxypropoxy]phenyl]-4-phenyl-1-piperazinehexanamideMaleate

A methanol solution of 4.10 g (0.0085 mol) of(R,S)-N-[4-[3-[(1-methylethyl)amino]-2-hydroxypropoxy]phenyl]-4-phenyl-1-piperazinehexanamideand 0.986 g (0.0085 mol) of maleic acid was concentrated and the residuewas recrystallized from acetonitrile-ethyl acetate to give the endproduct as colorless crystals, mp 144.5°-146.5°.

Anal. Calcd. for C₂₈ H₄₂ N₄ O₃.C₄ H₄ O₄ : C, 64.19; H, 7.74; N, 9.36.Found: C, 63.96; H, 7.74; N, 9.29.

EXAMPLE 78 11-Bromo-N-(4-hydroxyphenyl)undecanamide

A mixture of 94.7 g (0.357 mol) of 11-bromoundecanoic acid and 70 ml ofthionyl chloride was heated with a 90° oil bath until gas evolution hadessentially stopped. Excess thionyl chloride was distilled from thereaction mixture under aspirator vacuum. Toluene (50 ml) was added andthis was also distilled under aspirator vacuum. The cooled reactionmixture was then added to a slurry of 78.0 g (0.715 mol) of4-aminophenol in 500 ml of dioxane. After stirring for 30 min themixture was filtered and the solid was washed with hot dioxane. Thecombined filtrates were concentrated and the residue was recrystallizedfrom chloroform to give the end product as colorless crystals, mp116°-117°.

Anal. Calcd. for C₁₇ H₂₆ BrNO₂ : C, 57.30; H, 7.36; Br, 22.43; N, 3.93.Found: C, 57.25; H, 7.28; Br, 22.42; N, 4.17.

EXAMPLE 79 N-(4-Hydroxyphenyl)-4-phenyl-1-piperazineundecanamide

A solution of 66.8 g (0.187 mol) of11-bromo-N-(4-hydroxyphenyl)undecanamide, 30.3 g (0.187 mol) of1-phenylpiperazine and 27.0 ml (19.6 g, 0.194 mol) of triethylamine in600 ml of ethanol was heated under reflux overnight and then cooled. Theresulting solid was shaken with sodium bicarbonate solution anddichloromethane and the suspension was filtered. The solid wasrecrystallized from 95% ethanol to give the end product as colorlesscrystals, mp 160.5°-162.5°.

Anal. Calcd. for C₂₇ H₃₉ N₃ O₂ : C, 74.10; H, 8.98; N, 9.60. Found: C,74.28; H, 8.83; N, 9.42.

EXAMPLE 80(R,S)-N-[4-[3-[(1-Methylethyl)amino]-2-hydroxypropoxy]phenyl]-4-phenyl-1-piperazineundecanamide.

To a stirring solution of 14.0 g (0.20 mol) of potassium methoxide in400 ml of methanol was added 44.8 g (0.10 mol) ofN-(4-hydroxyphenyl)-4-phenyl-1-piperazineundecanamide. Fifteen minuteslater 90 ml of epichlorohydrin was added and stirring was continuedovernight. The reaction was concentrated under vacuum and the residuewas mixed with sodium bicarbonate solution and extracted withchloroform. The extracts were dried and concentrated and the solidresidue was recrystallized from trichloromethane and from ethyl acetateto give 34 g of a colorless solid. This was dissolved in 200 ml ofmethanol, 200 ml of 1-methylethylamine was added and the solution washeated under reflux overnight and then concentrated under vacuum. Theresidue was mixed with sodium bicarbonate solution and extracted withchloroform. The extracts were dried and concentrated to give a colorlesssolid. This was dissolved in chloroform and absorbed onto 500 g ofsilica gel. Elution with 1:1 methanol/chloroform gave material whichupon recrystallization from methanol gave(R,S)-N-[4-[3-[(1-methylethyl)amino]-2-hydroxypropoxy]phenyl]-4-phenyl-1-piperazineundecanamideas colorless crystals, mp 141.5°-142.5°.

Anal. Calcd. for C₃₃ H₅₂ N₄ O₃ : C, 71.70; H, 9.48; N, 10.13. Found: C,71.92; H, 9.56; N, 10.04.

EXAMPLE 81(R,S)-N-[4-[3-[(1-Methylethyl)amino]-2-hydroxypropoxy]phenyl]-4-phenyl-1-piperazineundecanamideMaleate

A mixture of 0.200 g (0.36 mmole) of(R,S)-N-[4-[3-[(1-methylethyl)amino]-2-hydroxypropoxy]phenyl]-4-phenyl-1-piperazineundecanamideand 42 mg (0.36 mmole) of maleic acid was recrystallized frommethanol-ethyl acetate to give the end product as colorless crystals, mp141°-142°.

Anal. Calcd. for C₃₃ H₅₂ N₄ O₃.C₄ H₄ O₄ : C, 66.44; H, 8.44; N, 8.38.Found: C, 66.59; H, 8.35; N, 8.28.

EXAMPLE 82(R,S)-N-[4-[3-[(1-Methylethyl)amino]-2-hydroxypropoxy]phenyl]-4-phenyl-1-piperazineundecanamideHydrochloride (1:2)

Hydrogen chloride was bubbled into a solution of 5.53 g (0.010 mol) of(R,S)-N-[4-[3-[(1-methylethyl)amino]-2-hydroxypropoxy]phenyl]-4-phenyl-1-piperazineundecanamidein a mixture of dichloromethane and chloroform. The mixture wasconcentrated under vacuum and the residue was recrystallized frommethanol-ethyl acetate to give the end product, mp 210°-212°.

Anal. Calcd. for C₃₃ H₅₂ N₄ O₃.2HCl: C, 63.34; H, 8.70; Cl, 11.33; N,8.95. Found: C, 63.27; H, 8.58; Cl, 11.34; N, 8.79.

EXAMPLE 83(R,S)-N-[4-[3-[(1-Methylethyl)amino]-2-hydroxypropoxy]phenyl]-4-phenyl-1-piperazinepropanamide.

To a rapidly stirring solution of 2.243 g (0.01 mol) of4-[2-hydroxy-3-[(1-methylethyl)amino]propoxy]benzenamine in 100 ml ofdichloromethane was added dropwise a solution of 0.80 ml (0.91 g, 0.01mol) of 2-propenoyl chloride. The reaction was stirred until theintermediate had settled out leaving a clear supernatant which wasdecanted. The residue was dissolved in 25 ml of ethanol, 5.0 ml (5.3 g,0.033 mol) of 1-phenylpiperazine was added, and the solution was heatedunder reflux overnight. The reaction mixture was cooled and filtered.The solid was dissolved in water, and the solution was made basic withsodium bicarbonate and extracted with dichloromethane. The extracts weredried and concentrated and the amorphous residue was reprecipitatedseveral times from ether to give the end product as a colorlessamorphous solid, mp 151°-157°. Analyses were performed on this amorphoussolid and subsequently the material was obtained crystalline andrecrystallized from ether to give the end product as colorless crystals,mp 250°-252°.

Anal. Calcd. for C₂₅ H₃₆ N₄ O₃ : C, 68.15; H, 8.24; N, 12.72. Found: C,68.33; H, 8.31; N, 12.65.

EXAMPLE 84

    ______________________________________                                        Tablet Formulations (Wet Formulation)                                          Ingredients        mg/tablet  mg/tablet                                      ______________________________________                                        (S)-1-[2-(4-(2-hydroxy-3-(1-methyl-                                                               25         50                                             ethyl)amino)-propoxy)phenoxy)ethyl]                                           4-phenylpiperazine bis-maleate salt                                           Pregelatinized Starch                                                                             12.5       15                                             Lactose             155        162                                            AVICEL              30         40                                             Modified Starch     25         30                                             Magnesium Stearate  2.5        3                                                                  250mg      300mg                                          ______________________________________                                    

Procedure:

Mix the first five ingredients in a suitable mixer. Granulate with waterand dry in an oven. Mill through a Fitzmill. Add the magnesium stearateand mix for 5 minutes and compress on a suitable tablet press.

EXAMPLE 85

    ______________________________________                                        Tablet  Formulation (Direct Compression)                                       Ingredients        mg/tablet  mg/tablet                                      ______________________________________                                        (S)-1-[2-(4-(2-hydroxy-3-(1-methyl-                                                               25         50                                             ethyl)amino)-propoxy)phenoxy)ethyl]                                           4-phenylpiperazine bis-maleate salt                                           Lactose             147.5      157                                            Modified Starch     25         30                                             AVICEL              50         60                                             Magnesium Stearate  2.5        3                                                                  250mg      300mg                                          ______________________________________                                    

The first four ingredients are blended in a suitable mixer. Themagnesium stearate is thereafter added and mixed for 5 minutes. Themixture is compressed on a suitable tablet press.

EXAMPLE 86

    ______________________________________                                        Capsule Formulation                                                            Ingredients        mg/capsule mg/capsule                                     ______________________________________                                        (S)-1-[2-(4-(2-hydroxy-3-(1-methyl-                                                               25         50                                             ethyl)amino)-propoxy)phenoxy)ethyl]                                           4-phenylpiperazine bis-maleate salt                                           Lactose             125        170                                            Cornstarch          40         60                                             Talc                15         20                                                                 250mg      300mg                                          ______________________________________                                    

The first three ingredients are blended in a suitable mixer andthereafter the talc is added. The mixture is blended for five minutesand filled on a suitable capsule machine.

EXAMPLE 87

Following the procedures outlined in Examples 84-86, the followingpreferred compounds and their pharmaceutically acceptable salts may beformulated into tablets or capsules:

(S)-4-[2-hydroxy-3-(1-methylethylamino)propoxy)-phenyl]-N-[2-(4-phenyl-1-piperazinyl)-ethyl]acetamide.

(S)-4-[2-hydroxy-3-(1-methylethylamino)propoxy)-phenyl]-N-[6-(4-phenyl-1-piperazinyl)-hexyl]acetamide.

(S)-1-[6-(4-hydroxy-3-isopropylaminopropoxy)-phenoxy)-hexyl]-4-phenylpiperazine.

What is claimed
 1. The compounds of the formulae ##STR41## wherein R₁ islower alkyl; R₈ is selected from the group consisting of --O--(CH₂)_(n)-wherein n is 2 to 20, ##STR42## and R₆ is selected from the groupconsisting of hydrogen or lower alkoxy, and ##STR43## wherein R₁ islower alkyl; R₈ is selected from the group consisting of --O--(CH₂)_(n)-wherein n is 2 to 20, ##STR44## and R₆ is selected from the groupconsisting of hydrogen or lower alkoxy, the racemates thereof and thepharmaceutically acceptable salts thereof.
 2. The compound of claim 1 ofthe formula ##STR45## wherein R₁ is lower alkyl; R₈ is selected from thegroup consisting of --O--(CH₂)_(n) -wherein n is 2 to 20, ##STR46## andR₆ is selected from the group consisting of hydrogen or lower alkoxy,the racemates thereof and the pharmaceutically acceptable salts thereof.3. The compound of claim 2 wherein R₈ is --O--(CH₂)_(n) -wherein n is 2to
 20. 4. The compound of claim 3 wherein n is 2 to
 11. 5. The compoundof claim 3 wherein n is 2 and R₆ is hydrogen.
 6. The compound of theformula ##STR47## wherein R₁ is lower alkyl, R₃ is selected from thegroup consisting of mesyl, tosyl, brosyl and benzenesulfonyl, R₆ ishydrogen or lower alkoxy and n is 2 to
 20. 7. The compound of claim 6wherein n is 2 to
 11. 8. The compound of claim 7 wherein n is
 2. 9. Thecompound of the formula ##STR48## wherein R₁ is lower alkyl, R₃ isselected from the group consisting of mesyl, tosyl, brosyl andbenzenesulfonyl, R₆ is hydrogen or lower alkoxy and n is 2 to
 20. 10.The compound of claim 9 wherein n is 2 to
 11. 11. The compound of claim10 wherein n is
 2. 12. The compound of the formula ##STR49## wherein R₁is lower alkyl and n is 2 to
 20. 13. The compound of claim 12 wherein nis 2 to
 11. 14. The compound of claim 13 wherein n is
 2. 15. Thecompound of the formula ##STR50## wherein R₁ is lower alkyl and R₆ ishydrogen or lower alkoxy; and the pharmaceutically acceptable saltsthereof.
 16. The compound of the formula ##STR51## wherein R₁ is loweralkyl, R₆ is hydrogen or lower alkoxy and n is 2 to 20, and thepharmaceutically acceptable salts thereof.
 17. The compound of claim 16wherein n is 2 to
 10. 18. The compound of claim 17 wherein n is
 2. 19.(S)-1-[2-(4-(2-Hydroxy-3-(1-methylethyl)aminopropoxy)phenoxy)ethyl]-4-phenylpiperazino,its racemate and the bismaleate salts thereof. 20.(S)-1-[2-(4-(2-Hydroxy-3-tert-butylaminopropoxy)phenoxy)ethyl]-4-phenylpiperazineand its bismaleate salt. 21.(S)-1-[2-(4-(2-Hydroxy-3-isopropylaminopropoxy)phenyl)ethyl]-4-(2-methoxyphenyl)piperazineand its bismaleate salt. 22.(S)-1-[6-(4-(2-Hydroxy-3-isopropylaminopropoxy)phenoxy)hexyl]-4-phenylpiperazineand its dihydrochloride salt. 23.(S)-1-[3-(2-(2-Hydroxy-3-isopropylamino-propoxy)phenoxy)propyl-4-phenylpiperazineand its dihydrochloride salt. 24.(S)-4-(2-Hydroxy-3-isopropylaminopropoxy)phenyl-N-[2-(4-phenyl-1-piperazinyl)ethyl]acetamide,its racemate and the dihydrochloride salts thereof. 25.(S)-4-(2-Hydroxy-3-isopropylaminopropoxy)phenyl-N-[6-(4-phenyl-1-piperazinyl)hexyl]acetamide,its racemate and the maleate salts thereof. 26.(R,S)-N[4-[3-[(1-Methylethylamino]-2-hydroxypropoxy]phenyl]-4-phenyl-1-piperazinepropanamideand its bismaleate salt.